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采用喷雾法研究了大黄酚对黄瓜白粉病的生物活性及其在黄瓜组织中的内吸传导性。结果表明,大黄酚对黄瓜白粉病菌具有较高毒力,感染白粉病的叶片经200 mg/L的大黄酚处理2 d后,霉状物变褐色,病斑枯死。扫描电子显微镜观察显示,黄瓜白粉病菌经大黄酚处理后,菌丝和分生孢子梗扭曲、变形,分生孢子塌陷。盆栽试验表明,利用大黄酚防治黄瓜白粉病具有较长的持效期,黄瓜子叶上喷施100 mg/L的大黄酚,20 d后接种病原菌,其防效仍达84.83%,与有效成分500 mg/L的硫磺悬浮剂防效相近。此外,大黄酚在黄瓜组织中具有一定的跨层传导性,其横向传导性较弱,几乎无向顶及向基的传导性。
The biological activity of chrysophanol against cucumber powdery mildew and its internal absorption conductivity in cucumber tissue were studied by spray method. The results showed that chrysophanol was highly virulent to powdery mildew of cucumber. After treated with 200 mg / L chrysophanol for 2 days, mildew was brown and spot was dead. Scanning electron microscopy showed that the cucumber powdery mildew was treated by chrysophanol, mycelium and conidiophores twisted, deformed, conidia collapse. Pot experiments showed that the use of chrysophanol control of cucumber powdery mildew has a longer persistence, cucumber cotyledons sprayed with 100 mg / L of chrysophanol, 20 d after inoculation of pathogens, the control effect still reached 84.83%, and the effective component 500 mg / L sulfur suspension similar control effect. In addition, chrysophanol has some cross-layer conductivity in cucumber tissue, its lateral conductivity is weak, and there is almost no conductivity to the top and to the base.