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将光敏核不育水稻农垦58S和正常可育农垦58N适时播种,使其光敏感期处于自然长日照控制下。于苗期、分蘖期、第二次枝梗分化期、雌雄蕊形成期、花粉母细胞形成期及减数分裂期分别在暗、光下取其幼穗及第一全展叶作蛋白质双向电泳分析,发现了17个特异的蛋白质组分。其中11个组分在可育与不育水稻间表现为有或无的差异;3个组分表现为表达量的变化;另3个组分表现为位置在双向电泳图谱上的平移。研究结果还表明低温和异常高温不仅可使光敏不育水稻的花粉育性出现部分恢复,而且高温处理还导致了电泳图谱上蛋白组分的相应改变。
Photosensitive nuclear sterile rice Nongken 58S and normal fertile Nongken 58N timely sowing, so that the light-sensitive period under the control of natural long sunshine. At the seedling stage, tillering stage, the second branch of stems differentiation stage, the formation of female and male pistils, the formation of pollen mother cells and meiosis were taken in the dark and light under the panicle and the first full-leaf protein two-dimensional electrophoresis Analysis, found 17 specific protein components. Among them, 11 components showed either or no difference between fertile and sterile rice; 3 components showed the change of expression; and the other 3 components showed the shift in the two-dimensional electrophoresis map. The results also showed that low temperature and abnormally high temperature not only partially restored the pollen fertility of photo-sensitive sterile rice, but also resulted in the corresponding changes of the protein components in the electrophoresis pattern.