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目的证实简捷—酶联免疫吸附试验试剂盒检测血吸虫病的特异度、灵敏度、交叉反应及现场应用价值。方法采用该试剂盒检测正常人，急性、慢性、晚期血吸虫病人，其他吸虫病人，以及血吸虫病不同流行程度的居民。结果该试剂盒的特异度为９７．４％，急性、慢性、晚期血吸虫病人的灵敏度分别为１００．０％、９３．２％和５２．０％，肺吸虫病的交叉反应为３３．７％，肝吸虫病为５．７％，姜片虫病无交叉反应。检测血吸虫病的阳性预测值、阴性预测值及诊断效率均在９５．０％以上。在血吸虫病轻、中、重度流行区的阳性检出率均显著地高于病原学检查。在血吸虫病新流行区查病，该试剂盒与ＩＨＡ符合率为９０．５％，阳性率差异无意义。结论简捷—酶联免疫吸附试验检测血吸虫抗体试剂盒特异性强、灵敏度高，在血吸虫病流行区交叉反应低，现场应用方便
Objective To confirm the simplicity - ELISA kit for detection of schistosomiasis specificity, sensitivity, cross-reaction and field value. Methods The kit was used to detect the residents of acute, chronic, advanced schistosomiasis, other trichomoniasis and schistosomiasis patients with different prevalences. Results The specificity of the kit was 97.4%. The sensitivity of acute, chronic and advanced schistosomiasis patients was 100.0%, 93.2% and 52.0% respectively. The cross-reaction rate of paragonimiasis was 33.7% , And hepatic fluke disease was 5.7%. There was no cross-reaction between ginger and schistosomiasis. The positive predictive value, negative predictive value and diagnostic efficiency of detecting schistosomiasis were more than 95.0%. In schistosomiasis mild, moderate and severe endemic positive detection rate was significantly higher than the etiological examination. In the endemic area of schistosomiasis, the coincidence rate of the kit and IHA was 90.5%, the positive rate difference was not significant. Conclusions The simple and sensitive enzyme-linked immunosorbent assay (ELISA) for detecting schistosoma japonicum antibody kit has high specificity, high sensitivity, low cross-reaction in schistosomiasis endemic area and convenient application on site