以蛇足石杉孢子囊为材料培养得到蛇足石杉原叶体,进一步对原叶体进行增殖培养并诱导产生蛇足石杉孢子体。结果表明,适合蛇足石杉原叶体增殖的培养基是无激素的MS培养基,在该培养基上培养60d,原叶体的增殖倍数达到131·1;较高浓度的外源激素对原叶体的增殖有抑制作用,而低浓度的外源激素对原叶体的增殖影响不大;原叶体在无激素培养基上培养90d,孢子体的诱导频率为4·0%;通过HPLC检测蛇足石杉原叶体中石杉碱甲的含量为0·0059%,是野生孢子体中含量的0·25倍。 The snake-footed Fusarium was obtained from the spores of the snake-footed Hedyotis fortunei, and the prothallia was further cultured and induced to produce the snake-footed Fusarium. The results showed that the medium suitable for the proliferation of Haemonchus obscurus was MS medium without hormone, and the multiplication of the original leaf body reached 131.1 after cultured on this medium for 60 days. The higher concentration of exogenous hormone on the original leaf While the low concentration of exogenous hormones had little effect on the proliferation of prothallium. The prothallium was cultured on hormone-free medium for 90 days and the induction frequency of sporophyte was 4.0% The content of huperzine A in the leaf of Haemonchus destructor was 0 · 0059%, which was 0.25 times of the content of wild spore.