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背景:成纤维细胞生长因子受体3与恶性肿瘤的关系已被证实,但是否在具有特殊生物学特性的牙源性肿瘤中扮演角色国内外文献报道较少。目的:观察成纤维细胞生长因子受体3在牙源性肿瘤中的表达。设计:随机对照观察。单位:湖州师范学院医学院口腔医学系,浙江大学医学院附属口腔医院。材料:实验于2003-01/2004-12在浙江大学口腔医学院完成。所有标本均来自浙江大学医学院附属口腔医院病理科1999～2003期间牙源性肿瘤病例,包括造釉细胞瘤29例,角化囊肿20例,始基囊肿36例,以5例正常牙囊或残余牙板上皮作为对照。所有标本均经石蜡包埋,切成3～5μm厚的切片,置于涂有多聚赖氨酸的载玻片上,烘干备用。方法:采用免疫组织化学方法,检测成纤维细胞生长因子受体3在正常牙囊或残余牙板上皮和牙源性造釉细胞瘤、角化囊肿及始基囊肿中的表达。主要观察指标:成纤维细胞生长因子受体3在造釉细胞瘤、角化囊肿、始基囊肿中的表达。结果:成纤维细胞生长因子受体3在造釉细胞瘤、角化囊肿及始基囊肿中呈阳性表达,表达率分别为59%、45%、8%,三者表达差异有显著性意义;在正常牙囊或残余牙板上皮中呈阴性表达。成纤维细胞生长因子受体3阳性细胞集中在肿瘤的细胞成熟区。结论:成纤维细胞生长因子受体3可能与造釉细胞瘤、角化囊肿的发病机制及终末分化机制有关。
BACKGROUND: The relationship between fibroblast growth factor receptor 3 and malignant tumors has been confirmed. However, whether it plays a role in odontogenic tumors with special biological characteristics has been reported in the literature both at home and abroad. Objective: To observe the expression of fibroblast growth factor receptor 3 in odontogenic tumors. Design: Randomized controlled observation. Unit: Department of Stomatology, Huzhou Teachers College, School of Stomatology, Zhejiang University School of Medicine. Materials: The experiment was performed at Zhejiang University School of Stomatology from January 2003 to December 2004. All samples were from the Stomatological Hospital of Zhejiang University School of Stomatology from 1999 to 2003, cases of odontogenic tumors, including ameloblastoma in 29 cases, keratinization in 20 cases, 36 cases of primordial cysts to 5 cases of normal dental follicles or Residual alveolar epithelium as a control. All specimens were paraffin-embedded, cut into 3 ~ 5μm thick slices, placed in polylysine coated glass slide, drying spare. Methods: Immunohistochemistry was used to detect the expression of fibroblast growth factor receptor 3 in normal or residual alveolar epithelium and odontogenic ameloblastoma, keratocysts and primordial cysts. MAIN OUTCOME MEASURES: Expression of Fibroblast Growth Factor Receptor 3 in ameloblastomas, keratocysts, primordial cysts. Results: The expression of fibroblast growth factor receptor 3 in ameloblastoma, keratocysts and primordial cysts was 59%, 45%, 8% respectively. There was significant difference among the three expressions. Negatively expressed in the normal or residual alveolar epithelium. Fibroblast growth factor receptor 3-positive cells are concentrated in the cell maturation zone of the tumor. CONCLUSION: Fibroblast growth factor receptor 3 may be related to the pathogenesis of terminal ameloblastoma and keratocyst and the mechanism of terminal differentiation.