随着造血干细胞研究的深入,对于粒系祖细胞(GM-CFUc)测试技术的研究和应用日趋广泛。在体外琼脂培养条件下,粒系祖细胞可以生成由粒系细胞、单核一巨噬细胞、或两者混合组成的细胞集落。它们的生成数量或产率(GM-CFUc/10~5)以及集落中的细胞组成,在一定程度上反映了机体的造血功能、集落刺激因子的性质以及这类细胞群体的不均一性的特征。本文介绍了我们在动物和人的骨髓细胞或外周血单个核细胞经体外琼脂培养后,对 With the deepening of research on hematopoietic stem cells, the research and application of GM-CFUc testing technology is becoming more and more widespread. Under in vitro agar culture conditions, myeloid progenitor cells can produce colonies of cells composed of myeloid cells, monocytes and macrophages, or a mixture of the two. Their numbers or yields of production (GM-CFUc / 10-5) and their cellular composition in colonies reflect to some extent the body’s hematopoietic function, the nature of colony stimulating factors, and the heterogeneity of such cell populations . This article describes our in vivo animal and human bone marrow cells or peripheral blood mononuclear cells after in vitro agar culture,