论文部分内容阅读
目的探讨 TNFα、IL- 6、IL- 4、IL- 2对 IFNγ诱导的小鼠腹腔巨噬细胞 ( mouse peritoneal maerophage,MPM)抗弓形虫 ( toxoplasm a gondii,TG)效应的机制。方法应用 3H- U标记的 TG在 MPM内的增殖实验及 1 2 5 I- Ud R标记的细胞毒实验 ,结果和结论 1高浓度 TNFα只能诱导 MPM轻微的抗 TG效应 ,而与亚剌激量 IFNγ结合几乎可以完全抑制 TG增殖 ;抗TNFα在 IFNγ诱导 MPM的最初阶段 ,可消除 IFNγ诱导的 ΜΡΜ 的抗 ΤG效应 ,与 ΙFNγ诱导 ΜΡΜ 产生内源性 ΤΝ Fα的作用相同。2 IL- 6可促进 TG在 MPM内的增殖 ,并能逆转 IFNγ诱导的 MPM抗 TG效应。3IL- 4在体外可部分抑制 TG在MPM内的增殖 ,其作用机制与 TNFα无关 ,但与 IFNγ有相加作用。4体外应用 IL- 2对 MPM抗 TG作用无影响 ;体内应用IL- 2明显延长急性 TG感染小鼠的生存时间 ( P<0 .0 5 ) ,治疗组小鼠脾细胞杀伤肿瘤细胞及 TG感染自身细胞的能力 ,均明显高于对照组 ( P<0 .0 0 1) ;而正常小鼠 NK细胞及 L AK细胞杀伤自身感染 TG靶细胞能力 ,显著低于杀伤肿瘤细胞的能力 ( P<0 .0 0 1) ;经 IL- 2诱导的 L AK细胞杀伤自身感染 TG靶细胞的能力 ,明显高于 NK细胞 ( P<0 .0 0 1) ,并与 L AK细胞杀伤肿瘤靶细胞的能力无明显差别 ( P>0 .0 5 )
Objective To investigate the mechanism of TNFα, IL-6, IL-4 and IL-2 on IFNγ-induced mouse peritoneal maerophage (MPM) toxoplasm a gondii (TG). Methods 3H-U-labeled TG in MPM proliferation experiments and 125 I-Ud R labeled cytotoxicity test results and conclusions 1 high concentrations of TNFα MPMP can only induce a slight anti-TG effect, and sub-stimulated Anti-TNFα abolished IFNγ-induced anti-TG effect of MPM during the initial phase of IFNγ-induced MPM, in the same manner as IGF-induced MPM production of endogenous TMFα by IFNγ. 2 IL-6 can promote the proliferation of TG in MPM, and can reverse IFNγ-induced anti-TG effect of MPM. 3IL-4 can partially inhibit the proliferation of TG in MPM in vitro, and its mechanism of action has nothing to do with TNFα, but with IFNγ added. In vitro application of IL-2 had no effect on the anti-TG effect of MPM. The in vivo application of IL-2 significantly prolonged the survival time of mice with acute TG infection (P <0.05). The spleen cells of mice in the treatment group killed tumor cells and TG infection (P <0.01). However, the ability of NK cells and L AK cells in normal mice to kill TG target cells was significantly lower than that in control cells (P < 0 0 01). The ability of L AK cells induced by IL-2 to kill TG target cells was significantly higher than that of NK cells (P <0.01 01) No significant difference in ability (P> 0.05)