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目的通过检测宫颈癌患者原发灶和血清中HPV DNA及其亚型,探讨其相关性及临床意义。方法选取24例行全子宫切除术或广泛全子宫切除术的宫颈癌患者的原发灶组织和术前静脉血,运用PCR方法对上述标本进行HPV DNA及亚型的检测。结果 (1)24例宫颈癌原发灶组织中HPV DNA阳性率为62.5%(15/24),以HPV16和HPV18两种亚型为主。24例血清标本中12例(50%)为HPV DNA阳性。血清HPV DNA阳性的患者中有7例(29.2%)所对应的原发灶HPV DNA为阳性并且两者亚型相同;3例(12.5%)患者所对应的原发灶HPV DNA为阳性但两者亚型不同;2例(8.3%)患者所对应的原发灶为HPV DNA阴性。(2)宫颈癌患者原发灶组织分化程度越低血清中HPV DNA检出率越高,与患者年龄、病理类型、临床分期无明显相关性。结论检测宫颈癌患者血清中HPV DNA并不能确切作为临床诊断宫颈癌的肿瘤标志物。宫颈癌患者血清中HPV DNA检出率与原发癌灶组织分化程度有关,与其年龄、病理类型、临床分期无明显相关性。
Objective To detect HPV DNA and its subtypes in primary tumor and serum of patients with cervical cancer, and to explore its correlation and clinical significance. Methods Twenty-four patients with cervical cancer undergoing hysterectomy or extensive hysterectomy were enrolled in this study. HPV DNA and subtype were detected by PCR. Results (1) The positive rate of HPV DNA in 24 cases of primary cervical cancer tissues was 62.5% (15/24), mainly HPV16 and HPV18 subtypes. Of the 24 serum samples, 12 (50%) were HPV DNA positive. Serum HPV DNA-positive patients, 7 (29.2%) corresponding to the primary HPV DNA was positive and the two subtypes were the same; 3 cases (12.5%) of the patients corresponding to the primary HPV DNA positive but two Subtypes were different; two cases (8.3%) of patients corresponding to the primary lesion is HPV DNA negative. (2) The lower the degree of differentiation of primary tumor in patients with cervical cancer, the higher the detection rate of HPV DNA in serum, and no significant correlation with age, pathological type and clinical stage. Conclusion The detection of HPV DNA in the serum of cervical cancer patients can not be used as a tumor marker for clinical diagnosis of cervical cancer. The detection rate of HPV DNA in the serum of cervical cancer patients is related to the differentiation degree of the primary foci, but not to the age, pathological type and clinical stage.