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枯草芽孢杆菌Bs-916是一种有效防治水稻病害的生防菌,为进一步提高其拮抗性能,获得更好的生防效果,运用离子注入(N+)的方法对Bs-916进行诱变,筛选到4株拮抗性能比出发菌Bs-916提高15%以上的菌株。TLC和HPLC定性、定量分析比较结果表明,Bs-916及其突变体分泌的脂肽类抗生素是表面活性素,突变菌株分泌的表面活性素比出发菌株有不同程度提高。室内抑菌结果表明,表面活性素能抑制纹枯病菌菌丝生长,引起原生质泄露。通过检测接种纹枯病菌、拮抗菌Bs-916及其突变菌株的水稻植株体内过氧化物酶(POD)、多酚氧化酶(PPO)和超氧化物歧化酶(SOD)酶活性的变化,发现拮抗菌Bs-916及其突变菌株分泌活性物质对水稻具有诱导抗性作用;4个高效突变株比出发菌Bs-916对3种酶的活性影响加大;同时接种拮抗菌和纹枯病菌比单独接种拮抗菌、纹枯病菌对3种酶活性影响大。
Bacillus subtilis Bs-916 is an effective biocontrol agent against rice diseases. To further improve its antagonistic properties and obtain better biocontrol effect, Bs-916 was mutated by ion implantation (N +) and screened To four antagonistic performance than the starting bacteria Bs-916 increased by more than 15% of the strains. The qualitative and quantitative analysis by TLC and HPLC showed that the lipopeptide antibiotics secreted by Bs-916 and its mutants were surfactant, and the surface active protein secreted by the mutant strains increased to some extent than the original strain. The results of indoor antibacterial test showed that surfactant could inhibit the mycelial growth of Rhizoctonia solani and cause the leakage of protoplasm. By detecting the changes of peroxidase (POD), polyphenol oxidase (PPO) and superoxide dismutase (SOD) activity of rice plants inoculated with Rhizoctonia solani and antagonistic bacteria Bs-916 and their mutants, It was found that the antagonistic bacteria Bs-916 and its mutant strains secreted active substances have induced resistance to rice; 4 high-efficiency mutant strains of Bs-916 than the starting bacteria on the activity of the three enzymes increased; at the same time inoculated with antagonistic bacteria and sheath blight Antagonistic bacteria than inoculated alone, Rhizoctonia solani on the three kinds of enzyme activity.