论文部分内容阅读
目的观察慢性阻塞性肺疾病(COPD)气道结构重塑的病理特征和气道慢性炎症的特点,探讨转化生长因子β1(TGF-β1)和结缔组织生长因子(CTGF)在COPD中的作用及其相互关系。方法将30只健康Wistar大鼠随机分为3组,正常对照组、盐水对照组和COPD组,通过气管内注射脂多糖和被动吸烟的方法建立COPD大鼠模型,检测支气管肺泡灌洗液(BALF)细胞计数及病理形态学等指标,采用免疫组化法检测肺组织TGF-β1和CTGF的表达。结果①BALF细胞总数:COPD组为(10.24±0.28)×108/L,正常对照组为(5.55±0.22)×108/L,两组比较差异有统计学意义(P<0.01);②肺泡腔直径COPD组为(91.71±1.56)μm正常对照组为(48.08±3.32)μm,两组比较差异有统计学意义(P<0.01);③免疫组化指数:COPD组的TGF-β1和CTGF表达分别为(3.65±0.58)分、(3.99±0.16)分,正常对照组分别为(0.80±0.19)分、(1.23±0.22)分,COPD组的TGF-β1和CTGF表达显著高于正常对照组(P<0.01)。结论多种炎性细胞参与COPD的发病;COPD气道重构的病理特征主要为小气道平滑肌不规则增生和细胞外基质沉积;TGF-β1和CTGF在COPD的发病中起重要作用,二者有高度相关性(r=0.938,P<0.01)。
Objective To observe the pathological features of airway remodeling and the characteristics of chronic airway inflammation in chronic obstructive pulmonary disease (COPD) and to explore the role of transforming growth factor β1 (TGF-β1) and connective tissue growth factor (CTGF) Interrelationship. Methods Thirty healthy Wistar rats were randomly divided into three groups: normal control group, saline control group and COPD group. COPD rat model was established by intratracheal instillation of lipopolysaccharide and passive smoking. Bronchoalveolar lavage fluid (BALF ) Cell count and pathological morphology and other indicators, immunohistochemical detection of lung TGF-β1 and CTGF expression. Results ① The total number of BALF cells was (10.24 ± 0.28) × 108 / L in COPD group and (5.55 ± 0.22) × 108 / L in normal control group, with significant difference between the two groups (P <0.01) (91.71 ± 1.56) μm in COPD group and (48.08 ± 3.32) μm in normal control group respectively. There was significant difference between the two groups (P <0.01). ③Immunohistochemical index: The expression of TGF-β1 and CTGF in COPD group were (3.65 ± 0.58) and (3.99 ± 0.16) points respectively, the levels of TGF-β1 and CTGF in COPD group were significantly higher than those in control group (0.80 ± 0.19) and (1.23 ± 0.22) P <0.01). Conclusions A variety of inflammatory cells are involved in the pathogenesis of COPD. The pathological features of airway remodeling in COPD mainly include small airway smooth muscle dysplasia and extracellular matrix deposition. TGF-β1 and CTGF play an important role in the pathogenesis of COPD. High correlation (r = 0.938, P <0.01).