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目的:了解鼠疫菌在历史鼠疫患者坟墓土壤中的存在状况。方法:采用赫氏培养基对鼠疫菌进行培养,同时利用Real-time定量荧光PCR对样本进行定性分析。结果:病原培养及荧光定量PCR定性分析显示样本均为阴性。结论:鼠疫菌在历史鼠疫患者坟墓的土壤中无法存活64年。
Objective: To understand the presence of Yersinia pestis in the grave soil of historical plague patients. Methods: Hepatic media was used to culture Y. pestis, and the samples were qualitatively analyzed by Real-time quantitative PCR. Results: Pathogen culture and quantitative PCR qualitative analysis showed that the samples were negative. Conclusion: Yersinia pestis can not survive 64 years in the soil of the historic plague patient’s grave.