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观察双氢青蒿素在不同浓度、不同时间对杜氏利什曼原虫（Ｌ．ｄ）汶川株及Ｌ．ｄ山东株前鞭毛体的体外作用。采用镜下观察及胸腺嘧啶核苷（３Ｈ－ＴｄＲ）掺入试验。结果：（１）镜下观察：实验组３个不同浓度（１４．１×１０－４ｍｏｌ／Ｌ、７．１×１０－４ｍｏｌ／Ｌ、３．５３×１０－４ｍｏｌ／Ｌ）的药物作用４８ｈ后，培养基颜色未变（红），虫体活动变慢以至几乎不动，虫数减少，抑制率升高（Ｌ．ｄ汶川株的抑制率由７３％升至８６％，Ｌ．ｄ山东株由６９．４％升至９７．５％），染色后见虫体变形，核、动基体不完整，胞质内出现多个空泡，鞭毛脱落。对照组的培养基颜色变黄，虫体活跃，大部分呈长梭形，并排成菊花状。染色后虫体呈长梭形，核、动基体、鞭毛、胞膜完整、清晰。（２）３Ｈ－ＴｄＲ掺入试验：双氢青蒿素在３．５３×１０－４ｍｏｌ／Ｌ２４ｈ即对两株有抑制作用（Ｌ．ｄ汶川株抑制率６５．８％，山东株为９３．４％）。但随着药物浓度的升高，作用时间的延长，抑制作用没有明显变化（Ｐ＞０．０５）。对Ｌ．ｄ山东株的抑制作用较Ｌ．ｄ汶川株为强。本文为首次报道双氢青蒿素对杜氏利什曼原虫具有较强的体外抑制作用。
Observation of dihydroartemisinin at different concentrations and different times on Leishmania donovani (L.d) Wenchuan strain and L. d in vitro Shandong promastigotes body function in vitro. Using microscopic observation and thymidine (3H-TdR) incorporation test. Results: (1) Under the microscope, three different concentrations of drugs (14.1 × 10-4mol / L, 7.1 × 10-4mol / L, 3.53 × 10-4mol / L) The color of the culture medium was unchanged (red), the activity of the parasites slowed down and almost did not move, the number of insects decreased and the inhibition rate increased (the inhibition rate of L.d strain increased from 73% to 86% Strain increased from 69.4% to 97.5%). After staining, the parasite was deformed, and nuclei and moving bodies were incomplete. A number of vacuoles appeared inside the cytoplasm and the flagella dropped off. The medium of the control group turned yellow, and the parasites were active. Most of them were long fusiform and arranged in a daisy pattern. After staining the fusiform spindle, nucleus, moving body, flagella, cell membrane integrity, clear. (2) 3H-TdR incorporation test: Dihydroartemisinin inhibited the two strains at 3.53 × 10-4mol / L24h (L.d strain was 65.8% and Shandong strain was 93%. 4%). However, with the increase of drug concentration, the prolongation of action time, the inhibition did not change significantly (P> 0.05). L d Shandong strains more inhibitory effect than L. d Wenchuan strong strains. This is the first report of dihydroartemisinin on Leishmania donovani has a strong in vitro inhibition.