用间接免疫酶方法在硝酸纤维膜上检查、筛选和回收重组痘苗病毒。作为人用疫苗株的筛选，此法较核酸杂交方法更简便、直观和准确，在筛选重组病毒的同时确定了病毒在感染细胞上的表达。构建了含痘苗病毒７．５ｋ启动子和ＥＢ病毒膜抗原基因的转移载体，从转染细胞的病毒混合液中用特异性ＭｃＡｂ和间接免疫酶方法直接筛选表达ＥＢ病毒膜抗原的重组痘苗病毒，并从阳性酶斑中回收具感染性病毒。 Indirect immunoenzymatic method on nitrocellulose membrane examination, screening and recovery of recombinant vaccinia virus. As a screening method for human vaccine strains, this method is more convenient, intuitive and accurate than the nucleic acid hybridization method. The virus was confirmed by screening the recombinant virus at the same time. The transfer vector containing the vaccinia virus 7.5k promoter and Epstein-Barr virus membrane antigen gene was constructed. The recombinant vaccinia virus expressing Epstein-Barr virus (EBV) membrane antigen was directly screened from the virus mixed solution of transfected cells by specific McAb and indirect immunoenzyme. The infectious virus was recovered from the positive plaques.