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研究基因功能的方法之一是减少或消除其在细胞中的表达。RNA干扰(RNA interference,RNAi)技术可以研究一个单独基因的作用,其效应因子为小干扰RNA(small interferingRNAs,siRNA)分子[1]。并非所有针对某基因不同位置碱基所设计的21个碱基siRNA都有相似基因沉默效率,针对同一mRN
One way to study the function of a gene is to reduce or eliminate its expression in a cell. RNA interference (RNAi) technology can study the role of a single gene, the effect factor for small interfering RNA (siRNA) molecules [1]. Not all 21 base siRNAs designed for different bases of a gene have similar gene silencing efficiency, and the same mRN