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以非洲菊(Gerber jamesonii)茎尖为初代培养材料,先去掉泥土及根,一个芽为一个外植体。切除叶,各芽留下最后未展开的心叶,切成2cm长,用小刀除去茎上暗咖啡色的外皮后,再切为1cm长的小段,用1%Naocl溶液浸泡10分钟,无菌水冲洗三次。消毒后培养的茎尖其大小为2-3mm。通过这些程序。茎尖初代培养的污染率能大幅度降低。继代培养的培养基由M_s(1962)的1/2量无机盐、全量有机物、BA5mg/1、IAA0.1mg/l、蔗糖2%和琼脂l%组成。pH5.6。每日16小时光照,强度为3000勒克斯。培养温度27~0士2℃。
Gerbera (Gerber jamesonii) stem tip for the initial culture material, first remove the soil and roots, a bud as a explant. Cut the leaves, each bud leaves the heart of the last unsplit leaf, cut into 2cm long, with a knife to remove dark brown skin on the stem, and then cut into 1cm long small section, soaked with 1% Naocl solution for 10 minutes, sterile water Rinse three times. Stem tip after disinfection culture its size is 2-3mm. Through these programs. Stem tip of the first generation of pollution can be significantly reduced. The subculture medium consisted of ½ amount of inorganic salts of M_s (1962), total organics, BA 5 mg / l, IAA 0.1 mg / l, sucrose 2% and agar 1%. pH 5.6. 16 hours daily light intensity 3000 lux. Cultivation temperature 27 ~ 0 disabilities 2 ℃.