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目的通过检测类风湿关节炎(RA)患者外周血单个核细胞(PBMC)、血浆中高迁移率族蛋白1(HMGB1)表达,为寻找治疗 RA 的新靶点提供依据。方法采集38例活动期 RA 患者、24例相对稳定期 RA 患者和20例健康对照者外周血。RT-PCR 检测 PBMC HMGB1mRNA 表达,Westernblot 检测 PBMC、血浆 HMGB1蛋白表达。结果与相对稳定期 RA 患者、健康对照者相比,活动期 RA患者 PBMC HMGB1mRNA 表达水平差异无统计学意义(F=1.23,P>0.05),而 HMGB1蛋白表达水平下降(F=70.91,P<0.01),血浆 HMGB1水平显著增高(P<0.001)。相对稳定期 RA 患者与健康对照者之间差异无统计学意义(P>0.05)。活动期 RA 患者血浆 HMGB1水平与 ESR(r_s=0.478,P<0.001)、C-反应蛋白(r_s=0.574,P<0.05)呈正相关。结论 HMGB1与 RA 发病密切相关,并可能成为新的治疗靶点。
Objective To detect the expression of high mobility group box 1 (HMGB1) in peripheral blood mononuclear cells (PBMC) and plasma in patients with rheumatoid arthritis (RA), so as to provide a basis for finding a new target for the treatment of RA. Methods 38 patients with active RA, 24 patients with stable RA and 20 healthy controls were collected. The expression of HMGB1 mRNA in PBMC was detected by RT-PCR. The expression of HMGB1 in PBMC and plasma was detected by Western blot. Results There was no significant difference in the expression of HMGB1 mRNA in PBMC between active RA patients and healthy controls (F = 1.23, P> 0.05), while the level of HMGB1 protein decreased (F = 70.91, P < 0.01), plasma HMGB1 levels were significantly higher (P <0.001). There was no significant difference between RA patients and healthy controls in relative stable period (P> 0.05). The plasma levels of HMGB1 in active RA patients were positively correlated with ESR (r_s = 0.478, P <0.001) and C-reactive protein (r_s = 0.574, P <0.05). Conclusion HMGB1 is closely related to the pathogenesis of RA and may be a new therapeutic target.