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目的:从表面活性剂中筛选出能逆转肿瘤细胞多药耐药(MDR)的耐药逆转剂。方法:用MTT法筛选出对多药耐药的细胞系K562／A02耐药的抗肿瘤药物,选择并确定表面活性剂没有细胞毒性的浓度,以维拉帕米作阳性对照,检测不同的表面活性剂在不同的浓度下逆转多药耐药的细胞系K562／A02对不同抗肿瘤药物耐药的能力。结果:硬脂酸聚氧乙烯、泊洛沙姆、蓖麻油聚氧乙烯醚、吐温-60、辛基酚聚氧乙烯醚等几种非离子表面活性剂均能降低抗肿瘤药物阿霉素、柔红霉素、长春新碱、依托泊苷、三尖杉酯碱对K562／A02细胞的IC50。结论:具有一定化学结构的非离子表面活性剂能逆转多药耐药的细胞系K562／A02的耐药性,作为药用辅料在逆转肿瘤多药耐药上将会有广阔的应用前景。
Objective: To screen out the drug-resistant reversal agents that can reverse the multidrug resistance (MDR) of tumor cells from surfactant. Methods: The antitumor drugs resistant to multidrug-resistant cell line K562 / A02 were screened by MTT method. The concentration of surfactant without cytotoxicity was selected and determined. Verapamil was used as a positive control to detect different surface The active agent reverses the multidrug resistance cell line K562 / A02 against different antitumor drugs at different concentrations. RESULTS: Several nonionic surfactants, such as polyoxyethylene stearate, poloxamer, polyoxyethylene ether of castor oil, Tween-60 and octylphenol polyoxyethylene ether, could decrease the antitumor drug doxorubicin , Daunorubicin, vincristine, etoposide, harringtonine on IC50 of K562 / A02 cells. Conclusion: Nonionic surfactant with certain chemical structure can reverse the drug resistance of multidrug resistant cell line K562 / A02. As a pharmaceutical excipient, it will have broad application prospect in reversing multidrug resistance of tumor.