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目的:探讨运动性中枢疲劳时大鼠大脑皮质MCT1、MCT2mRNA表达的变化以及乳酸对大鼠大脑皮质神经元MCT2mRNA表达的影响。方法:12只Wistar大鼠随机分为对照组和力竭训练1周组,每组6只。通过7天力竭训练建立中枢疲劳模型,观察中枢疲劳时大鼠大脑皮质MCT1、MCT2mR-NA表达变化;采用原代培养的大鼠大脑皮质神经元,观察不同浓度乳酸作用后大鼠大脑皮质神经元MCT2mRNA表达的变化。采用Trizol法提取大脑皮质和神经元总RNA;用SYBRAgreenI荧光定量RT-PCR测定MCT1及MCT2mRNA表达变化。结果:整体水平:力竭训练1周组MCT1mRNA表达无明显变化,而MCT2mRNA表达较对照组显著升高6·35±3·54倍(P<0·05)。细胞水平:乳酸作用后,神经元MCT2mRNA表达在pH=7·25时较对照组(pH=7·35组)升高3·08±2·38倍(P<0·05)。但随着乳酸浓度进一步升高,MCT2mRNA表达无明显增加。结果提示,中枢疲劳可能引发大脑皮质神经元MCT2mRNA表达升高,MCT2基因表达可能与乳酸在中枢疲劳中的作用有关。
Aims: To investigate the changes of MCT1 and MCT2 mRNA expression in rat cerebral cortex during exercise-induced central fatigue and the effect of lactate on the expression of MCT2 mRNA in rat cerebral cortical neurons. Methods: Twelve Wistar rats were randomly divided into control group and exhaustive training one week group with six rats in each group. After 7 days of exhaustive training, a model of central fatigue was established to observe the changes of MCT1 and MCT2mR-NA expression in rat cerebral cortex during central fatigue. Primary cultured rat cerebral cortical neurons were used to observe the effects of different concentrations of lactic acid on cerebral cortex nerve Meta-MCT2 mRNA expression changes. The total RNA of cerebral cortex and neurons was extracted by Trizol method. The expression of MCT1 and MCT2 mRNA was detected by SYBRAgreenI fluorescent quantitative RT-PCR. Results: The overall level of expression of MCT1 mRNA was not significantly changed in one week after exhaustive exercise training, while the expression of MCT2 mRNA was significantly increased by 6.35 ± 3.54 times (P <0.05). At the cellular level, the expression of MCT2 mRNA in neurons increased by 3.08 ± 2.38 folds (P <0.05) at pH = 7.25 compared with the control group (pH = 7.35) at the cellular level. However, with the further increase of lactate concentration, the expression of MCT2mRNA did not increase significantly. The results suggest that central fatigue may lead to increased expression of MCT2mRNA in cerebral cortex neurons, MCT2gene expression may be related to the role of lactate in central fatigue.