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目的 调查该院前列腺液培养结果及药敏情况 ,以协助临床前列腺炎的诊治。方法 回顾分析 2 2 5例前列腺液细菌培养结果及荧光标记 PCR技术检测淋球菌、沙眼衣原体、解脲脲原体 DNA的结果。结果 2 2 5份前列腺液 ,其中 14 1份细菌培养阳性 ,阳性率 6 2 .7% ,检出细菌 180株 ,革兰阳性菌 172株 ,革兰阴性杆菌 8株。荧光标记 PCR检测显示 ,淋球菌阳性 10份 ,沙眼衣原体阳性 4份 ,解脲脲原体阳性 2 8份。 116株葡萄球菌的体外药敏试验显示 ,对万古霉素、呋喃坦啶、利福平、复方新诺明敏感率较高 ,分别为 :10 0 %、97.3%、86 %、73%。 8株革兰阴性杆菌药敏结果显示 ,对亚胺培南、哌拉西林 /他唑巴坦、阿米卡星的敏感率较高 ,分别为 10 0 %、75 %、75 %。结论 凝固酶阴性葡萄球菌及棒状杆菌可能在前列腺炎致病中起一定作用 ,沙眼衣原体和解脲脲原体的作用有待探讨 ,细菌耐药性应引起广泛重视。
Objective To investigate the hospital prostatic fluid culture results and susceptibility to clinical prostatitis diagnosis and treatment. Methods The results of bacterial culture of 25 cases of prostatic fluid and DNA of Neisseria gonorrhoeae, Chlamydia trachomatis and Ureaplasma urealyticum were analyzed retrospectively. Results A total of 225 prostatic fluid samples were obtained from 14 1 bacterial cultures, with a positive rate of 6.27%. A total of 180 bacterial strains, 172 gram-positive bacteria and 8 gram-negative bacilli were detected. Fluorescent PCR detection showed that Neisseria gonorrhoeae positive 10, Chlamydia trachomatis positive 4, Ureaplasma urealyticum positive 28. In vitro susceptibility tests of 116 Staphylococcus aureus showed that the sensitivity to vancomycin, furadantin, rifampicin and cotrimoxazole was higher, which were 100%, 97.3%, 86% and 73% respectively. The susceptibility results of 8 Gram-negative bacilli showed that the susceptibility rates to imipenem, piperacillin / tazobactam and amikacin were higher, which were 10%, 75% and 75% respectively. Conclusion Coagulase-negative staphylococci and coryneform bacteria may play a role in the pathogenesis of prostatitis. The role of Chlamydia trachomatis and Ureaplasma urealyticum needs to be explored. Bacterial drug resistance should be given wide attention.