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目的观察直肠粘液腺癌组织中bax和bcl 2基因的表达并探讨其临床意义。方法应用免疫组织化学ABC法 ,以bax及bcl 2单克隆抗体标记 38例直肠粘液腺癌组织 ,并分析与临床病理学指标及预后的关系。结果bax的阳性表达率在DukesA和B期为 77 8% ,在DukesC期为45 0 % ,二者相比差异有显著意义 (χ2 =4 2 6 0 ,P =0 0 39) ;bcl 2的表达与各病理学指标无明显关系 ,但bax与bcl 2阳性表达强度之比值与Dukes分期 (P =0 0 4)和病人 5年生存率 (P =0 0 0 1)相关。结论bax及bcl 2在直肠粘液腺癌中普遍表达。术后检测癌组织中这类基因的表达对判断预后有积极作用
Objective To observe the expression of bax and bcl 2 gene in rectal mucinous adenocarcinoma and to explore its clinical significance. Methods 38 cases of rectal mucinous adenocarcinoma were labeled with bax and bcl 2 monoclonal antibodies by immunohistochemical ABC method and analyzed the relationship with clinicopathological parameters and prognosis. Results The positive expression rate of bax was 77 8% in Dukes A and B and 45 0% in Dukes C, the difference was significant (χ2 = 4260, P = 0 399); bcl2 There was no significant relationship between the expression of Bcl-2 and bcl-2 in Dukes stage (P = 0 0 4) and 5-year survival rate (P = 0 0 01). Conclusion bax and bcl2 are universally expressed in rectal mucinous adenocarcinoma. Postoperative detection of cancer tissue expression of these genes have a positive effect on the prognosis