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目的研究内皮素(ET)-1基因rs5370位点多态性与海南汉族老年脑梗死的关联性。方法选择海南省老年脑梗死患者150例作为实验组,健康对照156例作为对照组,提取基因组DNA,利用snapshot方法测定rs5370基因多态性。利用统计学软件分析组间差异。结果两组均可检测出三种基因型GG、GT、TT,三种基因型在实验组中的分布频率为79例(52.7%)、54例(36%)、17例(11.3%),在对照组中的分布频率为121例(68%)、47例(26.4%)、10例(5.6%),组间基因型分布频率存在明显差异(P<0.05)。等位基因G和T在实验组的分布频率为212例(70.7%)、88例(29.3%),在对照组中的分布频率为289例(81.2%)、67例(18.8%),组间差异显著(P<0.05)。结论 rs5370位点多态性与海南汉族脑梗死存在显著关联性,基因型GG可能是脑梗死保护因素,而等位基因T可能是海南汉族脑梗死的易感基因。
Objective To investigate the association between the polymorphism of rs5370 in endothelin (ET) -1 gene and elderly cerebral infarction in Han nationality in Hainan. Methods 150 elderly patients with cerebral infarction in Hainan Province were selected as experimental group and 156 healthy controls as control group. Genomic DNA was extracted and the rs5370 polymorphism was determined by snapshot method. Analysis of differences between groups using statistical software. Results Three genotypes of GG, GT and TT were detected in the two groups. The distribution frequency of the three genotypes in the experimental group was 79 cases (52.7%), 54 cases (36%) and 17 cases (11.3%), The frequency of distribution in the control group was 121 cases (68%), 47 cases (26.4%) and 10 cases (5.6%). There was a significant difference in genotype frequencies between the two groups (P <0.05). The distribution frequency of allele G and T in the experimental group was 212 cases (70.7%) and 88 cases (29.3%) in the experimental group, and 289 cases (81.2%) and 67 cases (18.8% The difference was significant (P <0.05). Conclusion There is a significant association between SNP rs5370 polymorphism and cerebral infarction in Hainan Han ethnicity. Genotype GG may be a protective factor against cerebral infarction, while allele T may be a susceptibility gene to cerebral infarction in Han nationality in Hainan.