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本文以多酚含量、总抗氧化能力(total antioxidant capacity,T-AOC)、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力和超氧化物歧化酶(superoxide dismutase,SOD)活性为测定指标,研究了不同光照和pH条件对药用真菌二型附毛孔菌Trichaptum biforme液体培养过程中抗氧化活性的影响。结果显示,二型附毛孔菌在液体培养过程中具有抗氧化活性。一定的光照可提高其分泌次级代谢产物的能力及抗氧化活性,14d光照条件下,多酚含量和T-AOC分别在第12天和第10天达到峰值,为1.85mg/m L和3.99U/m L;1d黑暗、1d光照交替的光照条件下,DPPH自由基清除率和SOD活性均于第2天达到最大值,分别为87.44%和0.63U/m L。不同pH条件对二型附毛孔菌液体培养过程中的抗氧化活性影响更大。当pH为9.0时,二型附毛孔菌在液体培养过程中分泌的多酚含量、T-AOC、SOD活性和DPPH自由基清除能力均最高,分别于第2天、第10天、第2天和第2天达到2.92mg/m L、5.22U/m L、0.70U/m L和95.07%。其次是pH 3.0,最后是pH 5.0和7.0,说明偏碱或偏酸条件更能刺激药用真菌二型附毛孔菌通过分泌大量次级代谢产物以抵抗来自微环境的氧化压力。
In this paper, polyphenol content, total antioxidant capacity (T-AOC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) Scavenging ability and superoxide dismutase (SOD) activity were measured to study the effects of different light and pH conditions on the anti-oxidative activity of the medicinal fungus Trichaptum biforme. The results showed that type Ⅱ attached to Pores have anti-oxidative activity in liquid culture. A certain amount of light could enhance its ability to secrete secondary metabolites and its antioxidant activity. Under 14 days of light, the contents of polyphenol and T-AOC peaked at 12 and 10 days respectively, which were 1.85 mg / m L and 3.99 U / m L; DPPH radical scavenging activity and SOD activity reached the maximum on the second day under 1d darkness and 1d light illumination, respectively, reaching 87.44% and 0.63U / m L, respectively. Different pH conditions have a greater impact on the anti-oxidative activity of the liquid culture of Type II with Pneumocystis. When the pH was 9.0, the polyphenol content, T-AOC, SOD activity and DPPH radical scavenging capacity secreted by Pneumocystis type II in liquid culture were the highest at the 2nd day, the 10th day and the 2nd day respectively And reached 2.92 mg / m L, 5.22 U / m L, 0.70 U / m L and 95.07% on the second day. Followed by pH 3.0 and finally pH 5.0 and 7.0, indicating that partial alkalisation or partial acidization can stimulate the medicinal fungi type-2-celled bacteria to secrete a large amount of secondary metabolites to resist oxidative stress from the microenvironment.