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目的 探讨肌苷对中枢神经再生的影响。方法 分离并制作均匀的视网膜神经节细胞 (RGC)悬液 ,采用 2 4孔培养皿培养 ,观察嘌呤类物质对RGC轴突生长及细胞存活的影响。结果 ( 1)肌苷促使RGC轴突生长 ;腺苷只有水解脱氨生成肌苷才能促使轴突生长。 ( 2 )肌苷与 6 硫鸟嘌呤 ( 6 TG)可能竞争性作用于蛋白激酶N(PKN)调节轴突生长。( 3 )肌苷刺激RGC致GAP 43表达增强。 ( 4 )肌苷的细胞内信息传递通路可能通过有丝分裂原激活蛋白激酶 (MAPK)、磷脂酰肌醇激酶 (PI3K)两条途径起作用。结论 肌苷在RGC神经再生中起积极的调节作用。
Objective To investigate the effect of inosine on CNS regeneration. Methods A uniform retinal ganglion cell (RGC) suspension was isolated and prepared. The cells were cultured in a 24-well culture dish to observe the effects of purine on axon growth and cell viability. Results (1) Inosine promoted RGC axon growth; adenosine promoted axon growth only by hydrolytic deamination to inosine. (2) Inosine and 6-thioguanine (6 TG) may compete with protein kinase N (PKN) to regulate axonal growth. (3) Inosine stimulated RGC induced GAP 43 expression. (4) The intracellular signaling pathway of inosine may play a role in the two pathways of mitogen-activated protein kinase (MAPK) and phosphatidylinositol kinase (PI3K). Conclusion Inosine plays a positive regulatory role in RGC nerve regeneration.