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目的 :检测乳腺癌患者骨髓微小转移灶 ,分析其与临床病期及远处转移的关系 ,并探讨其临床意义。 方法 :采用上皮膜抗原 ( EMA)单克隆抗体为探针 ,用免疫组化 ABC法检测乳腺癌患者骨髓中 EMA表达阳性细胞。结果 :58例乳腺癌患者有 3 3例骨髓中找到 EMA表达阳性细胞 ,阳性率为 57% ;晚期患者阳性细胞检出率显著增高 ;术后复发及有骨转移的患者较无复发及骨转移的患者阳性细胞检出率高 ;该方法与骨髓常规涂片检查比较 ,其阳性细胞检出率有显著差异。 结论 :骨髓中 EMA表达阳性细胞的检测可作为评价乳腺癌患者病情、了解有无复发及骨转移的重要指标之一。
Objective :To detect the micrometastasis of bone marrow in patients with breast cancer, analyze its relationship with clinical stage and distant metastasis, and explore its clinical significance. METHODS: EMA-positive cells in the bone marrow of breast cancer patients were detected by immunohistochemical ABC using epithelial membrane antigen (EMA) monoclonal antibody as a probe. RESULTS: EMA-expressing cells were found in 33 out of 58 breast cancer patients. The positive rate was 57%. The detection rate of positive cells in advanced patients was significantly higher. Patients with recurrence and bone metastases had no recurrence and bone metastases. The positive rate of positive cells in patients was high; this method was significantly different from the routine smear examination of bone marrow in the detection rate of positive cells. Conclusion: The detection of EMA-expressing cells in bone marrow can be used as one of the important indexes to evaluate the condition of breast cancer patients and to understand whether there is recurrence or bone metastasis.