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目的：以传统湿化学检测方法为参照，衡量干化学法检测部分心肌酶的有效性。方法依据美国国家临床实验室标准化委员会（NCCLS）EP9-A文件方案，每天随机选取临床标本8份，分别用干湿化学法测定样本天冬氨酸氨基转移酶（AST）、乳酸脱氨酶（LDH）、肌酸激酶（CK）活性单位，共测定6 d，记录结果，并以湿化学监测结果（x）为比较对象，对干化学法测定结果（y）进行评估。结果两种测定结果的回归方程为：AST，y=1.0623x+0.9656，r=0.9941；LDH， y=0.9988x-16.2818，r=0.9949；CK，y=0.9788x-3.8006，r=0.9988，并且随着三种酶活性的增加相对偏倚减小。结论干湿生化法测定AST、LDH、CK酶活性时，有很好的相关性，但在酶活性较低时测定结果的相对偏倚较大，因此，建议各临床实验室对不同方法建立不同的参考范围或者进行结果校正。“,”Objective To measure the effectiveness of some cardiac enzymes detected by dry chemistry method, wet chemistry method was taken as a reference. Methods Eight clinical specimens were randomly selected every day and it lasted six days, all the specimens were detected by wet and dry chemical methods. All of this was in accordance with the requirements of approved guideline, NCCLS, EP9-A. Results Three assays regression equations as following:AST, y=1.0623x+0.9656, r=0.9941; LDH, y=0.9988x-16.2818, r=0.9949;CK, y=0.9788x-3.8006, r=0.9988. And with the increasing of activity of these enzymes, the relative bias was decreased. Conclusion There was a good correlation between wet and dry chemical methods on AST, LDH and CK, however it showed a large bias at lower activity. And so, it was recommended to establish different reference ranges for different methods or to correct the results statistically.