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为了分析旋毛虫成囊前期幼虫（ＰＥＬ）抗原以寻找更有效的旋毛虫病诊断方法，本实验国内首次对ＰＥＬ的收集方法进行了研究。在贝氏法原理的基础上，分别采用了１６０目、２００目、２４０目和２８０目４种分样筛以及布袋收集ＰＥＬ，并观测了ＰＥＬ的数量和纯度。结果表明：在４种分样筛和各种收集时间中，用２４０目分样筛收集３ｈ为比较理想的实验条件，在此条件下能够得到大量纯度较高的ＰＥＬ虫体；另外，用棉布袋比用分样筛的收集效果更好。在收集２．５ｈ时用２４０目分样筛得到的虫体数量比用棉布袋多（ｐ＜０．０５），但纯度较低；在收集３ｈ以后，用这两种方法得到的虫体数量相近（Ｐ＞０．０５），而用布袋法得到的虫体纯度更高
In order to analyze the diagnosis of Trichinella spiralis pre-larvae (PEL) antigen in order to find a more effective method for the diagnosis of Trichinellosis, this study was the first to study the method of PEL collection in our country. Based on the principle of Bayesian method, PEL was collected from 4 kinds of sample sieves of 160 mesh, 200 mesh, 240 mesh and 280 mesh respectively and cloth bag, and the number and purity of PEL were observed. The results showed that in the four kinds of sample sieves and various collection time, with 240 mesh sieve collected 3h for the ideal experimental conditions, in this condition can get a large number of high purity PEL parasites; In addition, with cotton Bag than the use of sub-sieve collection better. The number of parasites obtained with the sieve of 240 mesh in 2.5h collection was higher than that of the cotton bag (p <0.05), but the purity was lower. After collecting for 3h, the number of parasites obtained by these two methods Similar (P> 0.05), while the bag obtained by bag method higher purity