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目的探讨脑出血后血肿周围组织细胞凋亡、Bcl-2和Bax蛋白表达与神经功能损伤的关系。方法SD大鼠70只,随机分为实验组和对照组,每组各35只。实验组采用胶原酶诱导尾状核脑出血模型,分别于术后6 h、12 h、24 h、48 h、72 h、7 d、14 d共7个时相点(每个时相点5只)评测其神经功能缺损情况。之后处死大鼠,采用TUNEL法、免疫组化SP技术,检测血肿周围脑组织细胞凋亡及Bcl-2,Bax蛋白的表达。结果脑出血后大鼠出现不同程度的神经功能缺损,以术后48 h左右损害严重,出血后6 h血肿周围组织出现TUNEL阳性细胞,48 h达高峰。Bcl-2和Bax蛋白表达高峰期分别是脑出血术后6 h和48 h。结论脑出血后细胞凋亡与神经损伤程度一致,细胞凋亡在脑出血后神经功能损伤中起重要作用。
Objective To investigate the relationship between apoptosis and expression of Bcl-2 and Bax proteins and neurological impairment in perihematomal cells after intracerebral hemorrhage. Methods Seventy-two SD rats were randomly divided into experimental group and control group with 35 rats in each group. The experimental group was induced by collagenase to induce the model of caudate nucleus hemorrhage, and were respectively subjected to 7 time points of 6 h, 12 h, 24 h, 48 h, 72 h, 7 d and 14 d after operation (5 Only) to evaluate the neurological deficit. Afterwards, the rats were sacrificed, and the apoptosis and expression of Bcl-2 and Bax proteins in brain tissue around the hematoma were detected by TUNEL and immunohistochemical SP technique. Results After the intracerebral hemorrhage, the rats showed different degrees of neurological deficits, with severe damage about 48 hours after operation. TUNEL positive cells appeared around the hematoma 6 hours after the hemorrhage and reached a peak at 48 hours. The peak of Bcl-2 and Bax protein expression was 6 h and 48 h after intracerebral hemorrhage respectively. Conclusions After ICH, the apoptosis is consistent with the degree of nerve injury. Apoptosis plays an important role in the neurological damage after intracerebral hemorrhage.