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具有高精度和高灵敏度的光镊技术在生物领域,特别是生物单分子力谱测量中,有着广泛和重要的应用.对微米量级粒子的稳定捕获是光镊技术开展应用研究的基础.非水环境在生物单分子研究领域越来越受到重视,本文对不同液体环境影响光阱稳定捕获的热力学因素进行研究,结合热力学仿真建立了热梯度力模型,分析了微球在向会聚光束焦点横向运动过程中受到的热梯度力的变化,并与T矩阵理论仿真得到的光阱力空间分布进行比较,总结了液体环境影响热梯度力的关键参数.在异丙醇环境里捕获直径为5μm硅球的实验中,5μm硅球无法进入光阱.针对这一实验现象,结合实验中微球的运动状态,通过分析仿真的光阱力和热梯度力在空间上的分布差异,给出了合理的解释,证明了上述理论的可行性,为评估不同溶液环境里激光热效应对光阱捕获微球的影响提供了分析方法.
Optical tweezers technology with high precision and high sensitivity has a wide range of applications in the field of biology, especially in the measurement of biological monomolecular forces.The stable capture of micrometer-sized particles is the basis for the application of optical tweezers technology. Water environment is paid more and more attention in the field of biological monomolecular research. In this paper, the thermodynamic factors affecting the steady trapping of light traps in different liquid environments are studied. The thermal gradient force model is established with thermodynamic simulation. And the thermal gradient force which is affected by the movement is compared with the spatial distribution of the light trapping force obtained from the T-matrix theory simulation.The key parameters of the thermal gradient force affecting the liquid environment are summarized.In the isopropanol environment, the diameter of 5μm silicon In the experiment of the ball, 5μm silica balls can not enter the light trap.According to the experimental phenomenon and the movement state of the microspheres in the experiment, the spatial distribution of the light trapping force and the thermal gradient force are analyzed, It proves the feasibility of the above theory and provides an analytical method for assessing the effect of laser thermal effects on optical trap trapped microspheres in different solution environments.