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目的观察rK39抗原酶联免疫吸附试验双抗原夹心法(ELISA夹心法)检测内脏利什曼病抗体用于内脏利什曼病诊断与宿主动物感染调查的可行性。方法采用ELISA夹心法与rK39免疫层析试条法(ICT)同步检测内脏利什曼病抗体。结果 5种家畜血清229份,7种鼠类标本238份,ELISA夹心法和rK39 ICT法抗体检测均阴性;接种利什曼原虫灰仓鼠、草原兔尾鼠标本33份,13份阳性,阳性率39.4%;塔里木兔标本119份,阳性7份,阳性率5.9%;非疫区儿童血清29份,全部阴性;疫区无内脏利什曼病症状人血清250份,4份两种方法均阳性,阳性率均为1.6%;住院内脏利什曼病病人血清67份,两种方法的阳性率分别为68.7%和67.2%。共检测人和其他动物标本965份,两种方法的阳性符合率98.6%,阴性符合率99.9%。ICT相同显色等级的阳性标本,ELISA跨10个滴度。结论 ELISA夹心法可检测多种动物内脏利什曼病抗体,抗体滴度具有定量意义。该方法适用于内脏利什曼病诊断、疗效观察和流行病学调查。
Objective To observe the feasibility of using rK39 antigen enzyme-linked immunosorbent assay (ELISA) to detect visceral leishmaniasis antibody in the diagnosis of visceral leishmaniasis and host animal infection. Methods The visceral leishmaniasis antibodies were detected by sandwich ELISA and rK39 immunochromatographic test strip (ICT). Results 229 serum samples of 5 kinds of livestock, 238 samples of 7 kinds of rodents were detected by ELISA sandwich method and rK39 ICT antibody test. 33 samples of Leishmania gray hamster and prairie rabbit were inoculated and 13 were positive 39.4%; 119 specimens of Tarim rabbit, positive 7, the positive rate of 5.9%; non-infected area of children with serum 29, all negative; no visceral leishmaniasis in infected areas of human serum 250, 4 were positive for both methods , The positive rate was 1.6%; 67 cases of visceral leishmaniasis in patients with serum, the positive rates of the two methods were 68.7% and 67.2%. A total of 965 specimens of human and other animals were examined. The positive coincidence rates of the two methods were 98.6% and the negative coincidence rates were 99.9%. ICT positive expression of the same color grade, ELISA across 10 titers. Conclusion ELISA sandwich method can detect a variety of animal visceral leishmaniasis antibodies, antibody titers have quantitative significance. The method is suitable for the diagnosis of visceral leishmaniasis, efficacy and epidemiological investigation.