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目的:本研究探索了梅毒抗体阳性标本经－20 ℃冻存后梅毒酶联免疫吸附试验(enzyme-linked immunosorbent assay，ELISA)检测方法的稳定性，并探索比较ELISA、化学发光微粒子免疫检测法(chemiluminescent microparticle immunoassay，CMIA)、甲苯胺红不加热血清试验(syphilis toluidine red untreated serum test，TRUST)与梅毒螺旋体明胶颗粒凝集试验(treponema pallidum gelatin particle agglutination test，TPPA)的诊断价值。方法:选取2014年3月至2015年3月我院通过ELISA法和(或)CMIA法初筛阳性标本483例血清标本，再用TPPA法检测。根据ELISA法检测的S/CO值将患者标本分别分为四组：A组(S/CO≥10)，B组(10>S/CO≥5)，C组(5>S/CO≥1)，D组(S/CO0.05)；B组标本冷冻前和冷冻后ELSIA方法检测结果与TPPA结果符合率差异无统计学意义(97.84%比97.84%，n P>0.05)；C组标本冷冻前和冷冻后ELSIA方法检测结果与TPPA结果符合率差异无统计学意义(91.11%比92.22%，n P>0.05)；D组标本冷冻前和冷冻后ELSIA方法检测结果与TPPA结果符合率差异有统计学意义(0%比88.89%，n P0.05)；而检测的冻存前标本ELISA法结果和CMIA法结果的敏感性分别为96.10%、99.57%，均高于TRUST敏感性(40.69%)，差异有统计学意义(n PS/CO ≥5), and group C(5>S/CO ≥1.0), group D(S/CO0.05). There was no significant difference in the coincidence rate between the results of ELSIA and TPPA in group B before and after freezing (97.84% vs 97.84%,n P>0.05). There was no significant difference in the coincidence rate between the results of ELSIA and TPPA before and after freezing in group C (91.11% vs 92.22%,n P>0.05). There was a statistically significant difference in the coincidence rate between the test results of ELSIA before and after freezing in group D (0% vs 88.89%,n P0.05). The sensitivities of ELISA and CMIA were 96.10% and 99.57% respectively, higher than TRUST sensitivity (40.69%), with statistically significant differences (n P<0.05).n Conclusion:Freezing at -20 ℃ had no effect on the samples positive for syphilis detected by ELISA, while it had some effect on the samples negative for syphilis detected by ELISA.False positive and false negative samples of ELISA, CMLA and TRUST methods should be paid attention to when detecting syphilis spirochetes antibody. The stability and accuracy of test results can be effectively guaranteed by using multi-method combined detection.