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目的对深圳市2004-2011年伤寒沙门菌进行脉冲场凝胶电泳(PFGE)分子分型及耐药性分析,为临床用药和追踪传染源提供依据。方法采用K-B法对42株伤寒沙门菌进行20种抗菌药物敏感性测试,并用PFGE对其进行分子分型。结果 42株伤寒沙门菌株对氨苄西林、阿莫西林/克拉维酸、氨苄西林/舒巴坦、头孢噻吩、头孢他啶、头孢曲松、头孢吡肟、头孢西丁、阿米卡星、庆大霉素、卡那霉素、环丙沙星、左旋氧氟沙星、复方新诺明、甲氧苄啶、氯霉素和四环素17种药物的敏感率均超过90%,对萘啶酸耐药率最高达61.5%。42株伤寒沙门菌株可分为SZ0001-SZ0028共28个PFGE型别,其中流行优势型为SZ0023型别。结论结合药物敏感试验结果和PFGE分子分型结果可以判断伤寒疫情的优势株型。
Objective To analyze the molecular typing and drug resistance of Salmonella typhimurium in Shenzhen City from 2004 to 2011 by pulse field gel electrophoresis (PFGE), and provide evidences for clinical use and tracing of infection sources. Methods Twenty-four strains of Salmonella typhi were tested for antimicrobial susceptibility using the K-B method and their molecular typing was performed using PFGE. Results 42 strains of Salmonella typhi were resistant to ampicillin, amoxicillin / clavulanic acid, ampicillin / sulbactam, cefalotin, ceftazidime, ceftriaxone, cefepime, cefoxitin, amikacin, The sensitivity of kanamycin, kanamycin, ciprofloxacin, levofloxacin, cotrimoxazole, trimethoprim, chloramphenicol and tetracycline were over 90% Rate up to 61.5%. 42 Salmonella typhi strains can be divided into SZ0001-SZ0028 total of 28 PFGE types, of which the predominant type is SZ0023 type. Conclusion The combination of drug sensitivity test results and PFGE molecular typing results can determine the predominant strains of typhoid fever.