光谱编码显微镜使用一个衍射光栅和光谱分析装置来获得显微图像。样品上不同的位置被不同的波长照明,通过对反射光光谱进行解码来得到空间信息。研制了一个基于扫频光源和平衡探测器的小型光谱编码显微镜(CSEM)。在没有放大器的情况下,一个固定增益的平衡探测器被用来探测较弱的样品光。通过对一个1951USAF分辨率测试靶成像来测量系统的横向分辨率。对离体猪小肠组织和在体手指皮肤成像来验证生物组织成像性能。结果表明,CSEM具有对生物组织深度分辨成像的能力。 Spectral-coded microscopes use a diffraction grating and spectroscopic analyzer to obtain microscopic images. Different locations on the sample are illuminated by different wavelengths, and the spatial information is obtained by decoding the reflected light spectrum. A compact spectroscopic coded microscope (CSEM) based on swept source and balanced detector was developed. In the absence of an amplifier, a fixed gain balanced detector is used to detect weaker sample light. The lateral resolution of the system was measured by imaging a 1951 USAF resolution test target. In vitro pig intestinal tissue and in vivo finger skin imaging to verify biological tissue imaging performance. The results show that CSEM has the ability to deeply discriminate biological tissues.