以红寺堡孙家滩园区2年定位试验土壤为研究对象,利用田间定位试验和BIOLOG技术相结合的方法,研究了三种不同培肥方式对荒漠草原土壤质量的影响。结果表明:施肥可以显著提高土壤养分含量,其中有机和无机配施效果最好,显著提高了土壤全氮、全磷、有机质、速效磷、速效钾和碱解氮含量;微生物数量和土壤微生物功能多样性增加。黄腐酸钾、羊粪和化肥配施处理的BIOLOG ECO板的平均颜色变化率斜率较高,增幅较快,土壤微生物群落对碳源利用能力较强;在培养96 h时与对照及施化肥处理有显著性差异。施化肥处理明显抑制了土壤微生物对碳源的利用能力。不同培肥方式下土壤微生物功能多样性主要受到土壤pH、碱解氮、全氮和土壤有机质的影响。有机肥、无机肥相结合的培肥方式能更好的提升土壤质量。 Taking the 2-year experimental soil of Sunjiatan Park in Hongsipu as research object, the effects of three different fertilizing modes on the soil quality of desert steppe were studied by field experiment and BIOLOG technology. The results showed that: fertilization can significantly increase the soil nutrient content, of which organic and inorganic compounding effect is the best, significantly increased soil total nitrogen, total phosphorus, organic matter, available phosphorus, available potassium and alkaline hydrolysis nitrogen content; microbial quantity and soil microbial function Diversity increases. The average color change rate of BIOLOG ECO treated with potassium humate, sheep manure and chemical fertilizers was higher than that of the control and chemical fertilizers at 96 h There are significant differences in treatment. Fertilization significantly inhibited the ability of soil microorganisms to utilize carbon sources. The functional diversity of soil microbes under different fertilization modes was mainly affected by soil pH, available nitrogen, total nitrogen and soil organic matter. Organic fertilizers, inorganic fertilizers combined with fertilizers can better improve soil quality.