论文部分内容阅读
用抗恶性疟原虫单克隆抗体13A2,制备兔抗血清,经BALB/c-IgG-Sepharose4B和13A2McAb-Sepharose4B柱提纯,得到抗独特型抗体(Ab2)。免疫双扩散试验和ELISA试验表明:抗独特型抗体仅与13A2McAb反应。4i试验显示:当恶性疟原虫抗原浓度为80μg/ml,对13A2Id-Antil3A2Id系统产生81%的抗体结合抑制率,而伯氏疟原虫和诺氏疟原虫抗原检测,抗体结合抑制率分别为27%和2.5%。兔抗血清中抗Ab2抗体(Ab3)滴度为1:640,提示此抗独特型抗体可替代原始抗原诱生抗恶性疟抗体。
Rabbit antiserum was prepared using anti-Plasmodium falciparum monoclonal antibody 13A2 and purified by BALB / c-IgG-Sepharose 4B and 13A2McAb-Sepharose 4B columns to give an anti-idiotypic antibody (Ab2). Immune double diffusion test and ELISA test showed that the anti-idiotypic antibody only reacted with 13A2McAb. The 4i assay showed that when P. falciparum antigen concentration was 80 μg / ml, 81% inhibition of antibody binding was produced on the 13A2Id-Antil3A2Id system, whereas the detection of Plasmodium berghei and Plasmodium nidulans against the binding of antibody was 27% And 2.5%. Rabbit antisera anti-Ab2 antibody (Ab3) titer of 1: 640, suggesting that anti-idiotypic antibodies can replace the original antigen-induced anti-falciparum malaria antibodies.