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目的测定金柴胡中总黄酮含量,并对其中三个黄酮成分进行薄层鉴别。方法采用薄层色谱法对金柴胡药材中的芦丁、槲皮素、异鼠李素进行了定性鉴别;采用紫外分光光度法测定不同产地金柴胡中总黄酮的含量。结果薄层鉴别斑点清晰,重复性好,金柴胡中总黄酮平均含量为3.993%。结论该方法简便可行,重复性好,可作为金柴胡药材的质量控制方法。
Objective To determine the content of total flavonoids in Bupleurum chinense and to identify the three flavonoids in them. Methods The contents of rutin, quercetin and isorhamnetin in Bupleurum chinense were identified by TLC. The content of total flavonoids in Jin Chaobu was determined by UV spectrophotometry. Results TLC spots clear, good repeatability, the average content of total flavonoids in Bupleurum was 3.993%. Conclusion The method is simple and feasible with good repeatability and can be used as a quality control method for Jin Bupleurum.