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目的 :探讨理想的喉癌生物治疗及联合治疗的新方法。方法 :利用裸鼠喉癌模型 ,通过不同的用药途径和模式观察新型重组人肿瘤坏死因子 (NrhTNF α)治疗喉癌裸鼠的疗效。并在光、电镜下观察NrhTNF α作用后肿瘤组织病理学及超微结构 ,探讨其作用机制。结果 :①喉癌裸鼠经NrhTNF α治疗后出现肿瘤出血、坏死 ,肿瘤缩小、消失 ,生存期延长。②NrhTNF α局部应用的抗肿瘤效果优于全身用药。③NrhTNF α与CTX联合应用具有增强抗瘤作用。④组织病理学及超微结构发现 ,NrhTNF α首先引起细胞质内线粒体、内质网肿胀及整个细胞空泡样变性 ,逐步导致核浓缩、崩解 ,最终致细胞死亡。结论 :NrhTNF α具有抗癌活性高、局部用药或联合用药效果更好 ,毒副作用少等优点 ,该研究为临床喉癌生物治疗及联合治疗提供了实验依据和理论基础。
Objective: To explore the ideal new method of biological treatment of laryngeal cancer and combination therapy. Methods: The nude mice model of laryngeal cancer was used to observe the therapeutic effect of the new recombinant human tumor necrosis factor (NrhTNF α) on laryngeal carcinoma in nude mice by different routes of administration and model. The histopathological and ultrastructural changes of tumor after NrhTNF α were observed under light and electron microscope to explore its mechanism. Results: ①Tumor hemorrhage, necrosis, shrinkage and disappearance of tumor occurred in nude mice with laryngeal cancer after NrhTNFα treatment, and prolonged survival. ② NrhTNF α topical application of anti-tumor effect is better than systemic medication. ③ NrhTNF α combined with CTX has anti-tumor effect. ④ Histopathology and ultrastructure showed that NrhTNF α firstly caused mitochondria and endoplasmic reticulum swelling in the cytoplasm and vacuolar degeneration of the whole cell, leading to nuclear condensation, disintegration and eventually cell death. Conclusion: NrhTNF α has the advantages of high anticancer activity, better local or combination therapy and less side effects. This study provides the experimental basis and theoretical basis for clinical treatment of laryngeal cancer and combination therapy.