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目的：系统探讨柯萨奇病毒Ｂ（ＣＶＢ）感染血症的机体免疫特征及干扰素应用的评价。方法：采用ＥＬＩＳＡ法测定ＣＶＢＡｇ和ＣＶＢＩｇＭ；采用单克隆抗体标记的间接ＡＢＣ免疫组化法测定Ｔ细胞亚群；３ＨＴｄＲ标记法测定ＮＫ细胞和ＬＡＫ细胞活性。结果：（１）ＣＶＢ感染机体ＮＫ细胞活性．ＬＡＫ细胞活性和ＣＤ４／ＣＤ８比值明显低于对照组；（２）机体ＮＫ和ＬＡＫ细胞活性轻中度降低，ＣＶＢＡｇ可自然清除或应用α干扰素清除；若ＮＫ和ＬＡＫ细胞活性严重降低，则ＣＶＢＡｇ清除慢，应用α干扰素也不能清除抗原。结论：α干扰素在ＣＶＢ感染中有应用价值。
Objective: To systematically investigate the immune characteristics of coxsackievirus B (CVB) in patients with sepsis and the evaluation of interferon application. Methods: The CVBAg and CVB IgM were determined by ELISA. T cell subsets were determined by indirect ABC immunohistochemistry with monoclonal antibody. The 3H-TdR labeling method was used to determine the activity of NK cells and LAK cells. Results: (1) CVB infection of NK cell activity. LAK cell activity and CD4 / CD8 ratio was significantly lower than that of the control group; (2) The activity of NK and LAK cells in the body decreased mildly and moderately, CVB-Ag could be cleared naturally or eliminated by interferon alfa; if the activities of NK and LAK cells were severely reduced, CVB Ag slow clearance, the application of interferon alpha can not clear the antigen. Conclusion: Alpha interferon has application value in CVB infection.