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继一氧化氮(NO)和一氧化碳(CO)之后,第三种气体信号分子硫化氢(H2S)对植物体生长发育和环境胁迫应答的调控正在受到越来越多的关注。钙离子(Ca2+)是重要的第二信使,参与植物对多种胁迫的响应。该实验以谷子这种抗逆性较强的作物为材料,对其响应六价铬(Cr6+)胁迫过程中H2S和Ca2+信号的互作进行了研究。结果表明,Cr6+胁迫显著激活谷子幼苗的H2S产生系统,外源H2S预处理能明显降低Cr6+胁迫对谷子根尖细胞的损伤,而H2S的合成抑制剂羟胺(HA)预处理,使得Cr6+对谷子的毒害增强;进一步实验发现,H2S能激活Ca2+信号下游相关基因的表达,同时Ca2+能增强H2S的产生,表明在植物体内H2S和Ca2+信号存在复杂的联系。该研究也证明,H2S和Ca2+可以通过调节重金属离子转运蛋白增强谷子对Cr6+的耐受。
Following the success of nitric oxide (NO) and carbon monoxide (CO), the regulation of the third gas signaling molecule, hydrogen sulfide (H2S) on plant growth and development and environmental stress responses is receiving more and more attention. Calcium (Ca2 +) is an important second messenger involved in plant responses to various stresses. In this experiment, the response of H2S and Ca2 + signals to Cr6 + stress was studied by using millet as a stress-tolerant crop. The results showed that Cr6 + significantly activated the H2S production system of millet seedlings. Pretreatment with exogenous H2S could significantly reduce the damage of root tip cells induced by Cr6 + stress. The pretreatment with hydroxylamine (HA), a synthetic inhibitor of H2S, Further experiments showed that H2S could activate the expression of related genes downstream of Ca2 + signal while Ca2 + could enhance the production of H2S, indicating that there is a complex relationship between H2S and Ca2 + signals in plants. The study also demonstrated that H2S and Ca2 + can enhance the tolerance of millet to Cr6 + by regulating heavy metal ion transporters.