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咖啡叶锈病是咖啡一种毁灭性病害。利用GRAMENE网站提供的SSR鉴定工具SSRIT(Simple Sequence Repeat Identification Tool),对咖啡叶锈病菌8 310条无冗余的EST分别进行SSR鉴定。结果共搜查到1 292个1~6碱基SSR,出现频率最高的为三碱基重复基元类型,其次为二碱基重复基元类型与六碱基重复基元类型。分别为459、321与214个,其出现频率分别为35.5%、24.8%与16.6%。AT/AT为二核苷酸中优势重复类型,占其总数的49.8%;而ATC/ATG与AAG/CTT则为三核苷酸中优势重复类型,二者分别占三核苷酸SSR总数的23.5%与23.3%。进一步对SSR多态性进行了预测,从而筛选出长度在22 bp以上具有潜在多态性的SSR 223个。此结果对于咖啡叶锈病菌群体遗传变异、多样性和进化等研究提供了分子标记。
Coffee leaf rust is a devastating disease of coffee. Using SSRIT (Simple Sequence Repeat Identification Tool) provided by the GRAMENE website, 8 310 non-redundant ESTs of the coffee leaf rust were identified by SSR. Results A total of 1 292 SSRs with 1 to 6 bases were found. The most frequently occurring SSRs were found in the three-base repeat motifs, followed by the two-base repeat motifs and six-base repeat motifs. 459, 321 and 214, respectively. The frequency of occurrence was 35.5%, 24.8% and 16.6% respectively. AT / AT is the dominant repeat type in dinucleotide, accounting for 49.8% of the total; while ATC / ATG and AAG / CTT are the dominant repeat types in trinucleotide, accounting for the total number of trinucleotide SSR 23.5% and 23.3%. The SSR polymorphism was further predicted, and 223 SSRs with potential polymorphism above 22 bp were screened out. The results provided a molecular marker for the research on genetic variation, diversity and evolution of coffee leaf rust population.