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Objective:To explore the influence of charred Gossamer urocteae(CGU) on the functions of primary cultured mouse oral fibroblasts and reveal its mechanism in wound healing.Methods:CGU was extracted with different solvents and ethanol extract(EE),ethyl acetate fraction(EF),n-butanol fraction(BF) and aqueous fraction(AF) were obtained.The effects of different fractions on the proliferation,matrix metalloproteinase-2,9(MMP-2,9) activities,synthesis of collagen and tissue inhibitor of metalloproteinase 1(TIMP-1) in the mouse oral fibroblasts were determined by MTT,gelatin zymography,chloramine-T method,and enzyme-linked immunosorbent assay(ELISA) respectively.Results:EE,EF and BF at high concentrations could significantly inhibit proliferation of fibroblasts(P<0.05 or P<0.01),and at low concentrations EF and BF could promote proliferation of fibroblasts,and BF and AF could significantly inhibit collagen synthesis(P<0.05 or P<0.01).EE,EF and AF at high concentrations could significantly increase the MMP-9 activity,and BF and AF could significantly inhibit synthesis of TIMP-1.Conclusion:CGU at high concentrations can inhibit the proliferations of fibroblasts and synthesis of collagen,and in healing of wound,CGU at high concentrations possibly has the functions of anti-fibrosis and anti-scar,and the mechanism to promote degradation of collagen is possibly related to the increase in MMP-9 activity and the inhibition of TIMP-1 synthesis.
Objective: To explore the influence of charred Gossamer urocteae (CGU) on the functions of primary cultured mouse oral fibroblasts and reveal its mechanism in wound healing. Methods: CGU was extracted with different solvents and ethanol extract (EE), ethyl acetate fraction (EF ), n-butanol fraction (BF) and aqueous fraction (AF) were obtained. These effects of different fractions on the proliferation, matrix metalloproteinase-2,9 (MMP-2,9) activities, synthesis of collagen and tissue inhibitor of metalloproteinase 1 (TIMP-1) in the mouse oral fibroblasts were determined by MTT, gelatin zymography, chloramine-T method, and enzyme-linked immunosorbent assay (ELISA) .Results: EE, EF and BF at high concentrations could significantly inhibit proliferation of (P <0.05 or P <0.01), and at low concentrations of EF and BF could promote proliferation of fibroblasts, and BF and AF could could inhibit compact synthesis (P <0.05 or P <0.01) concentrations could significantly incr ease the MMP-9 activity, and BF and AF could significantly inhibit synthesis of TIMP-1.Conclusion: CGU at high concentrations can inhibit the proliferations of fibroblasts and synthesis of collagen, and in healing of wounds, CGU at high concentrations may have the functions of anti-fibrosis and anti-scar, and the mechanism to promote the degradation of collagen is related to the increase in MMP-9 activity and the inhibition of TIMP-1 synthesis.