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目的:探讨姜黄素对人脊索瘤细胞系CM-319凋亡和放射敏感性的影响及作用机制。方法:体外培养CM-319细胞，分为姜黄素0 μmol/L组、姜黄素5 μmol/L组、姜黄素10 μmol/L组、姜黄素20 μmol/L组、si-NC组、si-UHRF1组、姜黄素+pcDNA组、姜黄素+pcDNA-UHRF1组，流式细胞仪检测细胞凋亡，Western Blot检测细胞中Bcl-2、Bax、Cleaved Caspase-3和UHRF1蛋白表达，qRT-PCR检测UHRF1 mRNA表达，克隆形成实验检测CM-319细胞对放射线敏感性。结果:姜黄素0 μmol/L组CM-319细胞凋亡率7.24%±0.73%，姜黄素5、10、20 μmol/L组CM-319细胞凋亡率分别为12.61%±1.57%、18.42%±1.54%、29.37%±2.65%，差异有统计学意义（n t=9.305；n t=19.680；n t=24.153，均n P<0.05）；Bcl-2蛋白相对表达量为0.76±0.07，余3组分别为0.62±0.06、0.51±0.05、0.33±0.03（n t=4.556；n t=8.719；n t=16.939，均n P<0.05）；Bax相对表达量为0.26±0.03、0.41±0.04、0.55±0.05、0.69±0.06（n t=9.000；n t=14.920；n t=19.230，均n P<0.05）；Cleaved Caspase-3相对表达量为0.24±0.03、0.37±0.04、0.49±0.05、0.62±0.06（n t=7.800；n t=12.862；n t=16.994，均n P<0.05），UHRF1mRNA相对表达量为1.01±0.09、0.82±0.08、0.67±0.07、0.42±0.04（n t=4.734；n t=8.946；n t=17.972，均n P<0.05），UHRF1蛋白相对表达量为0.83±0.08、0.61±0.06、0.48±0.05、0.31±0.0（n t=6.600；n t=11.130；n t=18.258，均n P<0.05），且呈剂量依赖性，增敏比分别为1.433、1.708和2.183。Si-NC组CM-319细胞中UHRF1蛋白相对表达量为0.79±0.08，si-UHRF1组表达降低为0.35±0.04（n t=14.758，n P<0.05）；CM-319细胞凋亡率分别为8.24%±0.83%和21.49%±2.11%（n t=17.531，n P<0.05）；Bcl-2蛋白表达水平分别为0.71±0.07、0.34±0.03（n t=14.575，n P<0.05）；Bax相对表达量为0.25±0.03、0.62±0.06（n t=16.547，n P<0.05）；Cleaved Caspase-3相对表达量为0.23±0.03、0.58±0.05（n t=18.007，n P<0.05），增敏比为1.727。姜黄素+pcDNA组CM-319细胞凋亡率为28.31%±3.01%，姜黄素+pcDNA-UHRF1组为13.59%±1.25%（n t=13.549，n P<0.05）；Bcl-2相对表达量分别为0.31±0.03、0.63±0.06（n t=14.311，n P<0.05）；Bax相对表达量分别为0.71±0.07、0.42±0.04（n t=10.791，n P<0.05）；Cleaved Caspase-3相对表达量分别为0.65±0.05、0.38±0.04（n t=12.650，n P<0.05），增敏比为0.539。n 结论:姜黄素可诱导CM-319细胞凋亡并增强CM-319细胞的放射敏感性，其机制与下调UHRF1表达有关。“,”Objective:To investigate the effects of curcumin on apoptosis and radiosensitivity of human chordoma cell line CM-319 and its mechanism.Methods:CM-319 cells were cultured in vitro and divided into curcumin 0 μmol/L group, curcumin 5 μmol/L group, curcumin 10 μmol/L group, curcumin 20 μmol/L group, si-NC group, si-UHRF1 group, curcumin+pcDNA group, curcumin+pcDNA-UHRF1 group.Flow cytometry was usedtodetect cell apoptosis, Western Blot was used to detectthe protein expression of Bcl-2, Bax, Cleaved Caspase-3 and UHRF1, qRT-PCR was used to detect the expression of UHRF1 mRNA, and clone formationexperiment was used to detect the sensitivity of CM-319 cells to radiation.Results:Compared with the curcumin 0 μmol/L group, the apoptosis rate of CM-319 cellsinthe curcumin 5 μmol/L group, curcumin 10 μmol/L group and curcumin 20 μmol/L groupincreased 12.61%±1.57%, 18.42%±1.54%, 29.37%±2.65% n vs 7.24%±0.73% (n t=9.305, 19.680, 24.153; all n P<0.05), and the protein expression of Bcl-2 decreased 0.62±0.06, 0.51±0.05, 0.33±0.03n vs 0.76±0.07 (n t=4.556, 8.719, 16.939; all n P<0.05), while the protein expression of Bax 0.41±0.04, 0.55±0.05, 0.69±0.06n vs 0.26±0.03 (n t=9.000, 14.920, 19.230; all n P<0.05) and Cleaved Caspase-3 0.37±0.04, 0.49±0.05, 0.62±0.06n vs 0.24±0.03 (n t=7.800, 12.862, 16.994; all n P<0.05) increased, and the expression of UHRF1 mRNA decreased 0.82±0.08, 0.67±0.07, 0.42±0.04n vs 1.01±0.09 (n t=4.734, 8.946, 17.972; all n P<0.05), the protein expression of UHRF1 decreased 0.61±0.06, 0.48±0.05, 0.31±0.03n vs 0.83±0.08 (n t=6.600, 11.130, 18.258; all n P<0.05), and were dose-dependent, and the sensitization ratios were 1.433, 1.708, and 2.183, respectively. Compared with the si-NC group, the protein expression of UHRF1in CM-319 cells in the si-UHRF1 group decreased 0.35±0.04n vs 0.79±0.08 (n t=14.758, n P<0.05), the apoptosis rate of CM-319 cells increased 21.49%±2.11%n vs 8.24%±0.83% (n t=17.531, n P<0.05), the protein expression of Bcl-2 decreased 0.34±0.03n vs 0.71±0.07 (n t=14.575, n P<0.05), while the protein expression of Bax 0.62±0.06n vs 0.25±0.03 (n t=16.547, n P<0.05) and Cleaved Caspase-3 0.58±0.05n vs 0.23±0.03 (n t=18.007, n P<0.05) increased, and the sensitization ratio was 1.727. Compared with the curcumin+pcDNA group, the apoptosis rate of CM-319 cells in the curcumin+pcDNA-UHRF1 groupreduced 13.59%±1.25%n vs 28.31%±3.01% (n t=13.549, n P<0.05), the protein expression of Bcl-2 increased 0.63±0.06n vs 0.31±0.03 (n t=14.311, n P<0.05), while the protein expression of Bax 0.42±0.04n vs 0.71±0.07 (n t=10.791, n P<0.05) and Cleaved Caspase-3 0.38±0.04n vs 0.65±0.05 (n t=12.650, n P<0.05) decreased, and the sensitization ratio was 0.539.n Conclusion:Curcumin can induce apoptosis of CM-319 cells and enhance the radiosensitivity of CM-319 cells, , and the mechanism is related to the down-regulation of UHRF1 expression.