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The isotherms of the interaction between the suspended particles and Cu2+,and the effects of lysine and asparaginic acid on the isotherms in the Huanghe (Yellow) River were studied by applying the theory and method of interfacial stepwise ion/coordination particle exchange. We obtained a new stepped river isotherm, formed by two curves joined together with a "plateau" in the middle. The adsorption equilibrium constants K1 and K2 were calculated by using the isothermal equation of surface stepwise ion exchange. Amino acid in small amount promotes exchange adsorption of the suspended particles with Cu2+. The degree of promotive action relates to the isoelectric point of amino acid. The promotive effect of lysine is bigger than that of asparaginic acid.