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本文应用微循环活体观察和荧光示踪技术，通过计算机数字图象处理对荧光素钠（ＦＩＮａ）和ＦＩＴＣ－Ｄｅｘｔｒａｎｓ（ＦＤ－４，ＭＷ４０００，ＦＤ－１５０，ＭＷ１５００００）在肠系膜微血管的通透过程进行了定量研究，建立了正常大鼠肠系膜微血管对不同分子量荧光物质的通透方程，得到了通透系数及对通透系数评价的定量方法。结果证实：颈动脉注射荧光素钠后４ｓ即可在微血管中见到，扩散速度很快，通透系数为３．３６２×１０－８ｃｍ２／ｓ。注射ＦＤ－４后１５ｓ可在微血管中见到，扩散速度比荧光素钠慢１倍左右，通透系数为１．７１８×１０－８ｃｍ２／ｓ。注射ＦＤ－１５０后８ｓ可在微血管中见到，扩散速度很慢，约为荧光素钠扩散速度的１／７０，通透系数为０．０４８６×１０－８ｃｍ２／ｓ。荧光物质的渗出部位主要在毛细血管及细静脉，在细动脉则极少见到。证实我们计算得出的通透系数能够较好地反映肠系膜微血管通透的实际情况，所采用的评价方法及得到的通透方程可以用于微血管物质交换参数的定量评价。
In this paper, microcirculation in vivo observation and fluorescent tracing technique were used to investigate the permeation of melanin (FINa) and FITC-Dextrans (FD-4, MW4000, FD-150 and MW150000) through mesentery capillaries through digital image processing A quantitative study was carried out to establish the permeability equation of normal rat mesenteric microvessels to different molecular weight fluorescent substances. The permeability coefficient and the quantitative method for evaluating the permeability coefficient were obtained. The results confirmed that: 4h after carotid artery injection of sodium fluorescein can be seen in the microvessels, diffusion rate is very fast, the permeability coefficient of 3.362 × 10-8cm2 / s. After injection of FD-4 15s can be seen in the capillaries, the proliferation rate of sodium fluorescein about 1 times slower, the permeability coefficient of 1.718 × 10-8cm2 / s. After injection of FD-150 8s can be seen in the microvascular, the diffusion rate is very slow, about 1/70 of the diffusion rate of sodium fluorescein, permeability coefficient of 0.0486 × 10-8cm2 / s. Exudation of fluorescent substances mainly in the capillaries and thin veins, rarely found in the arteries. Confirmed that the permeability coefficient we calculated can reflect the actual situation of mesenteric capillary permeability, the evaluation method and the permeability equation obtained can be used for the quantitative evaluation of microvascular exchange parameters.