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基于16S rDNA基因的文库构建和分析,通过对比分析了浑善达克和毛乌素沙漠中褐沙蒿根际细菌群落结构特点。结果表明:褐沙蒿根际细菌类群主要有9类,即芽单胞菌(Gemmatimonadetes)(4.26%/2.08%,浑善达克样/毛乌素沙样,下同)、酸杆菌(Acidobacteria)(2.15%/2.08%)、放线菌(Actinobacteria)(10.64%/8.33%)、拟杆菌(Bacteroidetes)(2.13%/4.17%)、绿弯菌(Chloroflexi)(0/2.08%)和α-变形菌(α-proteobacteria)(65.96%/56.25%)、β-变形菌(β-proteobacteria)(0/6.25%)、δ-变形菌(δ-proteobacteria)(2.08%/2.13%)和γ-变形菌(γ-proteobacteria)(10.64%/2.08%)。来自2个采样地的褐沙蒿根际细菌菌群结构总体相似,表明植物在此结构组成决定方面起了重要作用;两地根际细菌菌群最主要差别之一是毛乌素褐沙蒿根际细菌比浑善达克沙漠多了3种光合细菌,即红螺菌(Rhodospirillales)、红细菌(Rhodobacter)和绿弯菌。
Based on the library construction and analysis of 16S rDNA gene, the bacterial community structure characteristics of rhizosphere of Artemisia matsudana in Hunshandake and Mu Us Desert were compared and analyzed. The results showed that the rhizosphere bacteria population of Artemisia selengensis mainly includes 9 species, namely Gemmatimonadetes (4.26% / 2.08%, Hunshandake / Mu Us sand, the same below), Acidobacteria 2.15% / 2.08%), Actinobacteria (10.64% / 8.33%), Bacteroidetes (2.13% / 4.17%), Chloroflexi (0 / 2.08% Α-proteobacteria (65.96% / 56.25%), β-proteobacteria (0/6.25%), δ-proteobacteria (2.08% / 2.13% Γ-proteobacteria (10.64% / 2.08%). The rhizosphere bacteria assemblages of A. sativa from two sampling sites were generally similar, indicating that plants played an important role in determining the composition of the two cultivars. Bacteria have three more photosynthetic bacteria than the Hunshandake Desert, Rhodospirillales, Rhodobacter and.