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为了明确人工栽培桑黄(Phellinus spp.)的药用价值,以野生桑黄和人工栽培桑黄的子实体为材料,检测分析其水溶性活性物质粗多糖和粗酚的含量,以及其抗肿瘤、抗感染活性。采用乙醇浸提及甲醇萃取的方法提取栽培桑黄子实体粗酚的得率显著高于野生桑黄子实体,经超高效液相色谱-飞行时间质谱(UPLC-MS)技术分析2种桑黄子实体中的桑黄酚主要由丁香酸、原儿茶酸、原儿茶醛、咖啡酸4种单体酚组成。采用MTT法检测2种桑黄子实体来源粗多糖、粗酚以及4种单体酚物质对体外培养HCT116、HT-29、HepG 2肿瘤细胞的增殖均有较强抑制作用,2种来源的活性成分样品在相同剂量下的抑制作用差异不显著,其中原儿茶醛抑制肿瘤细胞增殖的活性较强。用流式细胞术体外检测桑黄粗酚中的单体酚物质对结肠炎患者外周血CD4+T细胞凋亡具有诱导活性,且咖啡酸、原儿茶酸抑制细胞增殖并诱导其凋亡的活性较强。研究结果表明,人工栽培桑黄子实体有良好的药用价值,其中多糖和原儿茶醛成分是抑制结肠癌、肝癌细胞增殖的主要活性物质,丁香酸、原儿茶酸通过诱导CD4+T细胞凋亡发挥抗感染作用。
In order to clarify the medicinal value of artificial cultivation of Phellinus spp., The contents of crude polysaccharide and crude phenol in water-soluble active substances were detected and analyzed by using the materials of wild Phellinus linteus and artificial fruiting body of Phellinus igniarius, , Anti-infective activity. The ethanol extraction and methanol extraction methods were better than those of P. morifolium. The results of UPLC-MS analysis of two kinds of Phellinus linteus The fruiting bodies of sulfanilamide is mainly composed of syringic acid, protocatechuic acid, protocatechuic aldehyde and caffeic acid. MTT assay was used to detect the proliferation of HCT116, HT-29 and HepG 2 tumor cells in vitro from two kinds of crude polysaccharides, crude phenol and four kinds of monomeric phenolics, respectively. The activities of the two sources There was no significant difference in the inhibitory effect of the component samples at the same dose, in which protocatechuic aldehyde inhibited the proliferation of tumor cells strongly. In vitro detection of monophenols in Phellinus igniarius by flow cytometry was used to induce the apoptosis of CD4 + T cells in peripheral blood of patients with colitis. And caffeic acid and protocatechuic acid can inhibit cell proliferation and induce apoptosis Strong activity. The results showed that the artificial cultivation of Phellinus linteus fruiting bodies had good medicinal value, of which polysaccharide and protocatechuic aldehyde were the main active substances inhibiting the proliferation of colon cancer and hepatocellular carcinoma cells. Syringholic acid and protocatechuic acid could induce CD4 + T Apoptosis play an anti-infective effect.