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以淮山为试材,采用毛细管电泳-电化学检测(CE-ED)方法测定淮山中3种植物甾醇(麦角甾醇、胆甾醇、β-谷甾醇)的含量,考察了检测电位、运行缓冲液浓度和pH、分离电压及进样时间等对检测效果的影响。结果表明:在优化的条件下,对0.5mg·L~(-1)麦角甾醇、胆甾醇、β-谷甾醇混合标准溶液连续6次进行测定在10min内实现了分离,线性范围分别为0.1~1 500、0.1~1 000、0.1~1 200μg·L~(-1),检出限分别为0.03、0.04、0.03μg·L~(-1),峰电流的相对标准偏差(RSD)分别为1.5%、1.7%、1.6%,迁移时间RSD分别为0.6%、0.7%、0.6%。该方法已用于淮山样品中胆甾醇、麦角甾醇和β-谷甾醇的测定,加标回收率在97.6%~100.3%,相对标准偏差(RSD)≤2.3%。
The contents of three phytosterols (ergosterol, cholesterol and β-sitosterol) in the yam were determined by Capillary Electrophoresis-Electrochemical Detection (CE-ED), and the detection potentials, running buffer concentration And pH, separation voltage and injection time on the test results. The results showed that under the optimal conditions, the standard solution of 0.5 mg · L -1 ergosterol, cholesterol and β-sitosterol was separated 6 times in a succession within 10 min, the linear range was 0.1 ~ The detection limits were 0.03, 0.04 and 0.03 μg · L -1, respectively, and the relative standard deviations (RSDs) of peak currents were respectively 1 500, 0.1-1 000 and 0.1-1 200 μg · L -1 1.5%, 1.7% and 1.6% respectively. The migration time RSD was 0.6%, 0.7% and 0.6% respectively. The method has been applied to the determination of cholesterol, ergosterol and β-sitosterol in yam samples with recoveries of 97.6% -100.3% and relative standard deviations (RSDs) ≤2.3%.