在法国Bordeaux大学和France’s INRA,M.Brison及其同事发现可用冷冻保存法消除实验污染的李属植物Prunus根状茎中的病毒。Brison等人特意用洋李痘病毒(Plum Pox Poty)感染离体生长的洋李根状茎。被污染植物的茎尖在加入了5%二甲亚砜和2%脯氨酸的预培养基中于4℃培养24小时,然后用经修饰的PVS2低温防护溶液处理并在浸入液氮前按1℃/mm冷却至-40℃。对照茎尖在分生组织培养基上培养不经冷冻。4个月后,检测茎尖中洋李痘病毒是否存在。作者报道,在一般情况下,20%的对照茎尖不存在病毒污染。小茎尖(0.3～0.5mm)的结果最好。相反,50%的冷冻保存植物没有病毒而且这一比例不受茎尖的大小影响。 Brison等人总结出:对于种质保存这是又一有用的方法,与单纯的分生组织培养相比冷冻 At Bordeaux University in France and France’s INRA, M. Brison and colleagues found that cryopreservation can be used to eliminate the experimentally contaminated Prunus rhizome virus from Prunus. In particular, Brison et al. Infected P. exserted rhizomes with Plum Pox Poty. The shoot tip of the contaminated plant was cultured in preincubation medium supplemented with 5% dimethylsulfoxide and 2% proline for 24 hours at 4 ° C and then treated with the modified PVS2 cryoprotective solution and before being immersed in liquid nitrogen 1 ℃ / mm cooled to -40 ℃. Control tips were cultured on mesenchymal media without freezing. Four months later, the presence of Plum-Pox virus in the shoot tips was examined. The authors report that in general, 20% of control stem tips are free of virus contamination. Small apical (0.3 ~ 0.5mm) the best results. In contrast, 50% of cryopreserved plants have no virus and this ratio is not affected by the size of the shoot tip. Brison et al. Concluded that this is yet another useful method for germplasm preservation, as compared to simple meristem culture