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目的考察大鼠ig芎汤超临界提取部位后,其有效成分藁本内酯、洋川芎内酯A、正丁基苯酞在大鼠体内的药动学规律。方法将芎汤超临界提取部位SD大鼠ig给药后,采集不同时间点的大鼠血浆样品,采用高效液相色谱法测定其中藁本内酯、洋川芎内酯A、正丁基苯酞的血药浓度,并采用PKSolver 2.0药动学软件计算其药动学参数,建立其药物代谢曲线。结果藁本内酯、洋川芎内酯A、正丁基苯酞分别在0.416~16.640、0.40~16.05、0.52~31.50μg/m L线性关系良好,回收率均大于84%,日内、日间精密度RSD均小于5%。藁本内酯主要药动学参数:t1/2为(2.082 0±0.637 6)h、Cmax为(3.272 5±0.955 5)μg/m L、AUC0~t为(15.400±1.812)μg·h/m L;洋川芎内酯A和正丁基苯酞的药动学参数与藁本内酯基本相似。结论建立的芎汤3种有效成分在大鼠血浆中的HPLC测定方法专属性强、灵敏度高,可用于大鼠血浆中藁本内酯、洋川芎内酯A、正丁基苯酞血药浓度的测定及药动学研究。
OBJECTIVE To investigate the pharmacokinetics of ligustilide, yangchongxiong A and n-butylphthalide in rats after the supercritical fluid extraction of ig xiongxiong decoction in rats. Methods After Xiongqiong decoction ig was administered to SD rats in supercritical fluid, the plasma samples of rats were collected at different time points. The contents of ligustilide, yangchongxiong lactone A, n-butylbenzene Phthalocyanine concentration, and using PKSolver 2.0 pharmacokinetic software to calculate the pharmacokinetic parameters, the establishment of its drug metabolism curve. Results The ligustilide, yangchongxiong A and n-butylphthalide showed good linearity in the range of 0.416 ~ 16.640,0.40 ~ 16.05 and 0.52 ~ 31.50μg / m L, respectively. The recoveries were all above 84% The degree of RSD is less than 5%. The main pharmacokinetic parameters of ligustilide were as follows: t1 / 2 was (2.082 ± 0.637 6) h, Cmax was (3.272 5 ± 0.955 5) μg / mL and AUC0 ~ t was (15.400 ± 1.812) μg · h / m L; Yang Chuanxiong lactone A and n-butylphthalide pharmacokinetic parameters and ligustilide are basically similar. Conclusion The established HPLC method for determination of three active ingredients of Xiongxiong Decoction in rat plasma has high specificity and high sensitivity, and can be used for the determination of ligustilide, yangchongxiong lactone A, n-butylphthalide, Determination of concentration and pharmacokinetic studies.