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溶血空斑实验是通过动物体内测定和筛选中药免疫抑制剂和增强剂的一种重复性好,较敏感而可靠的方法。实验药物为长寿灵复方(黄芪、黄精、刺五加、枸杞子、紫河车、首乌、玉竹)。实验用绵羊红细胞(SRBC)为我院动物室健康的绵羊,颈静脉采血置阿氏液中,放在冰箱保存备用。实验动物为LACA小白鼠30只,体重为20±2g,雌雄各半,随机分成三组,每组10只。第一、二组为实验组,每日胃灌含1:1生药“长寿灵”水煎剂1.0ml,用药一周,以第四天开始注射绵羊红细胞。第一组腹腔注射4×10~8/ml SRBC0.5ml;第二组尾静脉注射2×
Hemolysis plaque assay is a kind of repeatable, sensitive and reliable method for measuring and screening traditional Chinese medicine immunosuppressants and enhancers in animals. The experimental drug is Changshouling Compound (Huangqi, Huangjing, Ciwujia, Xunzi, Placenta, Shouwu, Yuzhu). Experimental sheep red blood cells (SRBC) were healthy sheep in the animal room of our hospital. Blood was taken from the jugular vein and placed in the refrigerator, and stored in the refrigerator for use. The experimental animals were 30 LACA mice, weighing 20±2g, male and female, and randomly divided into three groups of 10 mice each. The first group and the second group were experimental groups. The daily gastric lavage contained 1.0 ml crude drug “Changshouling” decoction and was administered for one week. The sheep red blood cells were injected on the fourth day. The first group was injected intraperitoneally with 4×10~8/ml SRBC 0.5ml; the second group was injected with 2× tail vein