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目的:探讨p63基因在喉鳞状细胞癌中的表达及其临床意义。方法:用免疫组织化学法检测63例喉鳞状细胞癌(喉癌组)及其45例癌旁组织(癌旁组)、24例转移淋巴结(转移淋巴结组)、40例未转移淋巴结(未转移淋巴结组)及25例喉非癌组织(非喉癌组)中p63基因的表达。结果:p63基因在喉鳞状细胞癌组织中均为细胞核阳性表达,喉癌组及转移淋巴结组的中、强阳性率为95.2%(60/63)和83.3%(20/24);癌旁组及未转移淋巴结组中无中、强阳性表达;非喉癌组中1例乳头状瘤为弱阳性表达,其余均为上皮细胞或基底细胞核弱阳性表达。结论:p63可作为喉鳞状细胞癌有特异性的标记物,对喉鳞状细胞癌的早期诊断和鉴别诊断有重要意义。p63可能主动参与了调控喉鳞状细胞癌的发生过程,但在喉鳞状细胞癌的发展过程中处于被激活状态,可能在发展过程中发挥负反馈的作用。
Objective: To investigate the expression of p63 gene in laryngeal squamous cell carcinoma and its clinical significance. Methods: Thirty-six laryngeal squamous cell carcinomas (LSCCs) and 45 paracancerous tissues (paracancer tissues) were detected by immunohistochemistry. Twenty-four lymph node metastases (lymph node metastasis) and 40 non-metastatic lymph nodes Metastatic lymph node group) and 25 cases of laryngeal non-cancerous tissue (non-laryngeal cancer group). Results: The positive rate of p63 gene in laryngeal squamous cell carcinoma was 95.2% (60/63) and 83.3% (20/24), respectively. Group and non-metastatic lymph node group had no positive expression. In non-laryngeal carcinoma group, 1 case of papilloma was weak positive expression, while others were weak positive expression of epithelial cells or basal cell nucleus. Conclusion: p63 can be used as a specific marker for laryngeal squamous cell carcinoma, which is of great significance for the early diagnosis and differential diagnosis of laryngeal squamous cell carcinoma. p63 may take part in the regulation of laryngeal squamous cell carcinoma, but it is activated in the process of laryngeal squamous cell carcinoma and may play a negative feedback role in the development.